ArticleJournal of immunology (Baltimore, Md. : 1950)2015
The unappreciated intracellular lifestyle of Blastomyces dermatitidis.
Article in Journal of immunology (Baltimore, Md. : 1950), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Homotypic interactions between SLAMF1 receptors on innate T cells and neutrophils regulate killing of fungi.PLoS pathogens · 2026Article
- Homotypic SLAMF1:SLAMF1 interactions between innate T cells and neutrophils activate fungal killing by neutrophils.bioRxiv : the preprint server for biology · 2026Article
- Brain-infiltrating CD4 T cells drive inflammatory microglia proliferation during cryptococcal meningitis in mice.Nature communications · 2025Article
- Nonoperative management for disseminated spinal blastomycosis with extensive lytic destruction in a 35-year-old male: illustrative case.Journal of neurosurgery. Case lessons · 2024Article
- A Rare Case of Uterine Blastomycosis and Its Management: A Case Report and Literature Review.Cureus · 2024Article
- Article
- Clinical Manifestations and Outcomes in Immunocompetent and Immunocompromised Patients With Blastomycosis.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Persistent and nonprogressive cutaneous blastomycosis in a pregnant adolescent.JAAD case reports · 2020Article
- Modulation of Immune Responses by Particle Size and Shape.Frontiers in immunology · 2020Review
- Monocytes and the Host Response to Fungal Pathogens.Frontiers in cellular and infection microbiology · 2020Review
- Turning on virulence: Mechanisms that underpin the morphologic transition and pathogenicity ofVirulence · 2019Review
- How Environmental Fungi Cause a Range of Clinical Outcomes in Susceptible Hosts.Journal of molecular biology · 2019Review
- Helper T-cell responses and pulmonary fungal infections.Immunology · 2018Review
- Incidental findings of blastomycosis lung nodules in five asymptomatic patients.Medical mycology case reports · 2018Article
- Lung Epithelial Cells Coordinate Innate Lymphocytes and Immunity against Pulmonary Fungal Infection.Cell host & microbe · 2018Article
- Metals in fungal virulence.FEMS microbiology reviews · 2018Review
- Clinical Manifestations and Treatment of Blastomycosis.Clinics in chest medicine · 2017Review
- Article
- Fungal Dimorphism and Virulence: Molecular Mechanisms for Temperature Adaptation, Immune Evasion, and In Vivo Survival.Mediators of inflammation · 2017Review
- Evidence of delayed dissemination or re-infection withMedical mycology case reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Blastomyces dermatitidis, a dimorphic fungus and the causative agent of blastomycosis, is widely considered an extracellular pathogen, with little evidence for a facultative intracellular lifestyle. We infected mice with spores, that is, the infectious particle, via the pulmonary route and studied intracellular residence, transition to pathogenic yeast, and replication inside lung cells. Nearly 80% of spores were inside cells at 24 h postinfection with 10(4) spores. Most spores were located inside of alveolar macrophages, with smaller numbers in neutrophils and dendritic cells. Real-time imaging showed rapid uptake of spores into alveolar macrophages, conversion to yeast, and intracellular multiplication during in vitro coculture. The finding of multiple yeast in a macrophage was chiefly due to intracellular replication rather than multiple phagocytic events or fusion of macrophages. Depletion of alveolar macrophages curtailed infection in mice infected with spores and led to a 26-fold reduction in lung CFU by 6 d postinfection versus nondepleted mice. Phase transition of the spores to yeast was delayed in these depleted mice over a time frame that correlated with reduced lung CFU. Spores cultured in vitro converted to yeast faster in the presence of macrophages than in medium alone. Thus, although advanced B. dermatitidis infection may exhibit extracellular residence in tissue, early lung infection with infectious spores reveals its unappreciated facultative intracellular lifestyle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.