Evidence map›Paper›PMID 25589024›Full record

ArticleScientific reports2015

Semi-automatic organelle detection on transmission electron microscopic images.

Takumi Higaki, Natsumaro Kutsuna, Kae Akita, Mayuko Sato, Fumie Sawaki, Megumi Kobayashi, Noriko Nagata, Kiminori Toyooka, Seiichiro Hasezawa

Abstract read
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Takumi HigakiDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa 277-8562, Japan.
Natsumaro Kutsuna1] Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa 277-8562, Japan [2] Research and Development Division, LPixel Inc., Bunkyo-ku, Tokyo 150-0002, Japan.
Kae AkitaDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa 277-8562, Japan.
Mayuko SatoRIKEN Center for Sustainable Resource Science, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Fumie SawakiFaculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan.
Megumi KobayashiFaculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan.
Noriko NagataFaculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan.
Kiminori ToyookaRIKEN Center for Sustainable Resource Science, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Seiichiro HasezawaDepartment of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa 277-8562, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent advances in the acquisition of large-scale datasets of transmission electron microscope images have allowed researchers to determine the number and the distribution of subcellular ultrastructures at both the cellular level and the tissue level. For this purpose, it would be very useful to have a computer-assisted system to detect the structures of interest, such as organelles. Using our original image recognition framework CARTA (Clustering-Aided Rapid Training Agent), combined with procedures to highlight and enlarge regions of interest on the image, we have developed a successful method for the semi-automatic detection of plant organelles including mitochondria, amyloplasts, chloroplasts, etioplasts, and Golgi stacks in transmission electron microscope images. Our proposed semi-automatic detection system will be helpful for labelling organelles in the interpretation and/or quantitative analysis of large-scale electron microscope imaging data.

Indexed as

AutomationImage Processing, Computer-AssistedMicroscopy, Electron, TransmissionArabidopsisCells, CulturedCotyledonGolgi ApparatusMitochondriaNicotianaOrganellesPlastids

Identifiers

PMID25589024
PMCPMC4295107

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.