ArticleProtoplasma2015
Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues.
Article in Protoplasma, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
- Advances in Imaging of Plant CaBiomolecules · 2026Review
- Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy.Scientific reports · 2025Article
- Live cell imaging of plant infection provides new insight into the biology of pathogenesis by the rice blast fungus Magnaporthe oryzae.Journal of microscopy · 2025Review
- Article
- Signaling at Physical Barriers during Pollen-Pistil Interactions.International journal of molecular sciences · 2021Review
- Advances in Two-Photon Imaging in Plants.Plant & cell physiology · 2021Review
- ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging.Plant & cell physiology · 2021Article
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube.Plant reproduction · 2021Article
- Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis.PLoS biology · 2021Article
- A Cationic Porphyrin, ZnPor, DisassemblesAntibiotics (Basel, Switzerland) · 2020Article
- Host-parasite tissue adhesion by a secreted type of β-1,4-glucanase in the parasitic plant Phtheirospermum japonicum.Communications biology · 2020Article
- Intracellular dynamics and transcriptional regulations in plant zygotes: a case study of Arabidopsis.Plant reproduction · 2020Review
- Imaging flowers: a guide to current microscopy and tomography techniques to study flower development.Journal of experimental botany · 2020Review
- Polyspermy Block in the Central Cell During Double Fertilization ofFrontiers in plant science · 2020Article
- Live-Cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2020Article
- ANTH domain-containing proteins are required for the pollen tube plasma membrane integrity via recycling ANXUR kinases.Communications biology · 2018Article
- Article
- Cell-cell communications and molecular mechanisms in plant sexual reproduction.Journal of plant research · 2018Review
- Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines.Protoplasma · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo imaging of living organisms is an important tool to investigate biological phenomena. Two-photon excitation microscopy (2PEM) is a laser-scanning microscopy that provides noninvasive, deep imaging in living organisms based on the principle of multiphoton excitation. However, application of 2PEM to plant tissues has not been fully developed, as plant-specific autofluorescence, optically dense tissues, and multiple light-scattering structures diminish the clarity of imaging. In this study, the advantages of 2PEM were identified for deep imaging of living and intact Arabidopsis thaliana tissues. When compared to single-photon imaging, near-infrared 2PEM, especially at 1000 nm, reduced chloroplast autofluorescence; autofluorescence also decreased in leaves, roots, pistils, and pollen grains. For clear and deep imaging, longer excitation wavelengths using the orange fluorescent proteins (FPs) TagRFP and tdTomato gave better results than with other colors. 2PEM at 980 nm also provided multicolor imaging by simultaneous excitation, and the combination of suitable FPs and excitation wavelengths allowed deep imaging of intact cells in root tips and pistils. Our results demonstrated the importance of choosing both suitable FPs and excitation wavelengths for clear two-photon imaging. Further advances in in vivo analysis using 2PEM will facilitate more extensive studies in the plant biological sciences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.