ArticleJournal of clinical microbiology2015
Development and validation of a quantitative PCR assay using multiplexed hydrolysis probes for detection and quantification of Theileria orientalis isolates and differentiation of clinically relevant subtypes.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- From Benign Parasite to Emerging Pathogen: A Review ofAnimals : an open access journal from MDPI · 2026Review
- Morphological Examination and Molecular Characterization of Theileria spp. From Dairy Cattle in Lingwu, Ningxia, China.Acta parasitologica · 2026Article
- The efficacy of toltrazuril for prevention of oriental theileriosis (Theileria orientalis Ikeda genotype) in Friesian calves.Australian veterinary journal · 2025Article
- Development and validation of a real-time SYBR green PCR method for the detection and differentiation of Babesia and Theileria species (Apicomplexa: Piroplasmida) in hard ticks and cattle blood from Thailand.Parasite (Paris, France) · 2025Article
- Article
- A comprehensive molecular survey of vector-borne blood parasites in cattle in Kyrgyzstan with a note of the first molecular detection of Anaplasma bovis and Candidatus Anaplasma Camelii.Tropical animal health and production · 2024Article
- Epidemiology, Clinical Signs, and Risk Factors Associated with Theileriosis in Australian Cattle (2006-2022).Pathogens (Basel, Switzerland) · 2024Article
- Article
- Epidemiology and Transmission ofPathogens (Basel, Switzerland) · 2023Review
- Distribution and Prevalence ofPathogens (Basel, Switzerland) · 2023Article
- Distribution ofPathogens (Basel, Switzerland) · 2022Article
- Characterisation of thePathogens (Basel, Switzerland) · 2022Article
- Complete Genomes ofPathogens (Basel, Switzerland) · 2022Article
- Molecular identification of Theileria spp. in ruminants and ticks from southern littoral of Caspian Sea, Iran.Tropical animal health and production · 2022Article
- Coinfection of cattle in Virginia withJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- Approaches to Integrated Parasite Management (IPM) forPathogens (Basel, Switzerland) · 2021Review
- Effects ofPathogens (Basel, Switzerland) · 2021Article
- Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific.Microorganisms · 2021Article
- A U.S. isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis.Parasites & vectors · 2021Article
- Molecular detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Theileria orientalis is an emerging pathogen of cattle in Asia, Australia, and New Zealand. This organism is a vector-borne hemoprotozoan that causes clinical disease characterized by anemia, abortion, and death, as well as persistent subclinical infections. Molecular methods of diagnosis are preferred due to their sensitivity and utility in differentiating between pathogenic and apathogenic genotypes. Conventional PCR (cPCR) assays for T. orientalis detection and typing are laborious and do not provide an estimate of parasite load. Current real-time PCR assays cannot differentiate between clinically relevant and benign genotypes or are only semiquantitative without a defined clinical threshold. Here, we developed and validated a hydrolysis probe quantitative PCR (qPCR) assay which universally detects and quantifies T. orientalis and identifies the clinically associated Ikeda and Chitose genotypes (UIC assay). Comparison of the UIC assay results with previously validated universal and genotype-specific cPCR results demonstrated that qPCR detects and differentiates T. orientalis with high sensitivity and specificiy. Comparison of quantitative results based on percent parasitemia, determined via blood film analysis and packed cell volume (PCV) revealed significant positive and negative correlations, respectively. One-way analysis of variance (ANOVA) indicated that blood samples from animals with clinical signs of disease contained statistically higher concentrations of T. orientalis DNA than animals with subclinical infections. We propose clinical thresholds to assist in classifying high-, moderate-, and low-level infections and describe how parasite load and the presence of the Ikeda and Chitose genotypes relate to disease.
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