ArticleVirulence2015
Establishment of an oral infection model resembling the periodontal pocket in a perfusion bioreactor system.
Article in Virulence, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Three dimensional (3D) gingival models in periodontal research: a systematic review.Journal of materials science. Materials in medicine · 2023Pooled it
- 3D models for oral inflammation using gingival fibroblasts, lipopolysaccharides and hypoxia.Scientific reports · 2026Article
- Advances in oral disease models: a mini-review of developments from 2015 to 2025.Frontiers in dental medicine · 2026Review
- Engineered Tissue Models to Decode Host-Microbiota Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- The proteome of osteoblasts in a 3D culture perfusion bioreactor model compared with static conditions.Scientific reports · 2025Article
- An in vitro model demonstrating homeostatic interactions between reconstructed human gingiva and a saliva-derived multispecies biofilm.Microbiome · 2025Article
- Advances in modeling periodontal host-microbe interactions: insights from organotypic and organ-on-chip systems.Lab on a chip · 2025Review
- Cytokine release and NLRP3 inflammasome activation induced by low-abundance oral bacterial biofilms.Journal of oral microbiology · 2025Article
- Biofabrication Strategies for Oral Soft Tissue Regeneration.Advanced healthcare materials · 2024Review
- A 3D In-vitro model of the human dentine interface shows long-range osteoinduction from the dentine surface.International journal of oral science · 2024Article
- Microfluidic organ-on-chip systems for periodontal research: advances and future directions.Frontiers in bioengineering and biotechnology · 2024Review
- Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis.Journal of oral microbiology · 2024Article
- Clinical, oral immunological and microbiological shifts during and after pregnancy.Clinical oral investigations · 2023Observational
- Microphysiological Modeling of Gingival Tissues and Host-Material Interactions Using Gingiva-on-Chip.Advanced healthcare materials · 2023Article
- A physiologically relevant culture platform for long-term studies of in vitro gingival tissue.Acta biomaterialia · 2023Article
- Three-Dimensional Humanized Model of the Periodontal Gingival Pocket to Study Oral Microbiome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A high-throughput, 28-day, microfluidic model of gingival tissue inflammation and recovery.Communications biology · 2023Article
- A novel in vitro periodontal pocket model to evaluate the effect of root surface instrumentation on biofilm-epithelial cell interactions.Clinical oral investigations · 2022Article
- Amphiregulin promotes hair regeneration of skin-derived precursors via the PI3K and MAPK pathways.Cell proliferation · 2021Article
- Immunoresponsive Tissue-Engineered Oral Mucosal Equivalents Containing Macrophages.Tissue engineering. Part C, Methods · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Periodontal infection involves a complex interplay between oral biofilms, gingival tissues and cells of the immune system in a dynamic microenvironment. A humanized in vitro model that reduces the need for experimental animal models, while recapitulating key biological events in a periodontal pocket, would constitute a technical advancement in the study of periodontal disease. The aim of this study was to use a dynamic perfusion bioreactor in order to develop a gingival epithelial-fibroblast-monocyte organotypic co-culture on collagen sponges. An 11 species subgingival biofilm was used to challenge the generated tissue in the bioreactor for a period of 24 h. The histological and scanning electron microscopy analysis displayed an epithelial-like layer on the surface of the collagen sponge, supported by the underlying ingrowth of gingival fibroblasts, while monocytic cells were also found within the sponge mass. Bacterial quantification of the biofilm showed that in the presence of the organotypic tissue, the growth of selected biofilm species, especially Campylobacter rectus, Actinomyces oris, Streptococcus anginosus, Veillonella dispar, and Porphyromonas gingivalis, was suppressed, indicating a potential antimicrobial effect by the tissue. Multiplex immunoassay analysis of cytokine secretion showed that interleukin (IL)-1 β, IL-2, IL-4, and tumor necrosis factor (TNF)-α levels in cell culture supernatants were significantly up-regulated in presence of the biofilm, indicating a positive inflammatory response of the organotypic tissue to the biofilm challenge. In conclusion, this novel host-biofilm interaction organotypic model might resemble the periodontal pocket and have an important impact on the study of periodontal infections, by minimizing the need for the use of experimental animal models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.