Evidence map›Paper›PMID 25587426›Full record

ArticleBiosensors2014

Biosensor Applications of MAPLE Deposited Lipase.

Valeria Califano, Francesco Bloisi, Antonio Aronne, Stefania Federici, Libera Nasti, Laura E Depero, Luciano R M Vicari

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Valeria CalifanoIstituto Motori-CNR, via G. Marconi 8, 80125 Napoli, Italy.
Francesco BloisiDepartment of Physics, University of Naples "Federico II", Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Napoli, Italy; E-Mails: bloisi@na.infn.it (F.B.); lidar@na.infn.it (L.N.); vicari@unina.it (L.R.M.V.) ; SPIN-CNR, c/o Dipartimento di Fisica, Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Napoli, Italy.
Antonio AronneDepartment of Chemical Engineering, Materials and Industrial Production, University of Naples "Federico II", Piazzale V. Tecchio 80, 80126 Napoli, Italy; E-Mail: anaronne@unina.it.
Stefania FedericiDIMI-Dipartimento di Ingegneria Meccanica ed Industriale, University of Brescia, via Branze 38, 25123 Brescia, Italy; E-Mails: stefania.federici@unibs.it (S.F.); laura.depero@unibs.it (L.E.D.).
Libera NastiDepartment of Physics, University of Naples "Federico II", Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Napoli, Italy; E-Mails: bloisi@na.infn.it (F.B.); lidar@na.infn.it (L.N.); vicari@unina.it (L.R.M.V.).
Laura E DeperoDIMI-Dipartimento di Ingegneria Meccanica ed Industriale, University of Brescia, via Branze 38, 25123 Brescia, Italy; E-Mails: stefania.federici@unibs.it (S.F.); laura.depero@unibs.it (L.E.D.).
Luciano R M VicariDepartment of Physics, University of Naples "Federico II", Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Napoli, Italy; E-Mails: bloisi@na.infn.it (F.B.); lidar@na.infn.it (L.N.); vicari@unina.it (L.R.M.V.) ; SPIN-CNR, c/o Dipartimento di Fisica, Complesso Universitario di Monte Sant'Angelo, via Cintia, 80126 Napoli, Italy.
University of Naples Federico II · ITUniversity of Brescia · ITIstituto Motori · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Matrix Assisted Pulsed Laser Evaporation (MAPLE) is a thin film deposition technique derived from Pulsed Laser Deposition (PLD) for deposition of delicate (polymers, complex biological molecules, etc.) materials in undamaged form. The main difference of MAPLE technique with respect to PLD is the target: it is a frozen solution or suspension of the (guest) molecules to be deposited in a volatile substance (matrix). Since laser beam energy is mainly absorbed by the matrix, damages to the delicate guest molecules are avoided, or at least reduced. Lipase, an enzyme catalyzing reactions borne by triglycerides, has been used in biosensors for detection of β-hydroxyacid esters and triglycerides in blood serum. Enzymes immobilization on a substrate is therefore required. In this paper we show that it is possible, using MAPLE technique, to deposit lipase on a substrate, as shown by AFM observation, preserving its conformational structure, as shown by FTIR analysis.

Indexed as

biosensorslipaseMAPLE

Identifiers

PMID25587426
PMCPMC4287705
OpenAlexW2036970296

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.