ArticleParasitology2015
Identification of Toxoplasma gondii antigens associated with different types of infection by serum antibody profiling.
Article in Parasitology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Seroprevalence and Genotype Distribution ofHealth science reports · 2026Article
- Recombinant ROP6 protein expressed in Saccharomyces cerevisiae INVSc1 cells induced strong immune response and provided significant protection against toxoplasmosis.Scientific reports · 2025Article
- Limited value of current and newFrontiers in parasitology · 2023Article
- Comparative Performance of Recombinant GRA6, GRA7, and GRA14 for the Serodetection ofPathogens (Basel, Switzerland) · 2022Article
- Identification of Oocyst-DrivenMicroorganisms · 2021Review
- Development of a hexavalent recombinant protein vaccine adjuvanted with Montanide ISA 50 V and determination of its protective efficacy against acute toxoplasmosis.BMC infectious diseases · 2020Article
- Detection of antibodies against Toxoplasma gondii in cats using an immunochromatographic test based on GRA7 antigen.The Journal of veterinary medical science · 2020Article
- Study on Circulating Antigens in Serum of Mice With Experimental Acute Toxoplasmosis.Frontiers in microbiology · 2020Article
- Key Limitations and New Insights Into theFrontiers in cellular and infection microbiology · 2020Review
- Discovery of new Toxoplasma gondii antigenic proteins using a high throughput protein microarray approach screening sera of murine model infected orally with oocysts and tissue cysts.Parasites & vectors · 2018Article
- Article
- Development of ELISAs for diagnosis of acute typhoid fever in Nigerian children.PLoS neglected tropical diseases · 2017Article
- Distinct antibody responses of patients with mild and severe leptospirosis determined by whole proteome microarray analysis.PLoS neglected tropical diseases · 2017Article
- Towards Development of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays.Journal of clinical microbiology · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Acquisition of acute toxoplasmosis during the first trimester of pregnancy can have catastrophic consequences for the foetus. Diagnosis is routinely based on the detection of maternal Toxoplasma gondii--antibodies using whole parasite extracts as detection antigen. While such assays are sensitive, they show no specificity for the stage of infection. We hypothesized diagnosis might be improved using recombinant antigens for detection, particularly if antibodies to certain antigen(s) were associated with early or late stages of infection. To address this, protein microarrays comprising 1513 T. gondii exon products were probed with well-characterized sera from seronegative ('N') controls, and acute ('A'), chronic/IgM-persisting ('C/M') and chronic ('C') toxoplasmosis cases from Turkey. Three reactive exon products recognized preferentially in A infections, and three recognized preferentially in C/M infections, were expressed in Escherichia coli and tested for discrimination in IgG ELISAs. The best discriminators were exon 1 of TGME49_086450 (GRA5) which detected C/M infections with 70.6% sensitivity and 81.8% specificity, and exon 6 of TGME49_095700 (ubiquitin transferase domain-containing protein) which detected A infections with 84.8% sensitivity and 82.4% specificity. Overall, the data support a recombinant protein approach for the development of improved serodiagnostic tests for toxoplasmosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.