Evidence map›Paper›PMID 25586463›Full record

ArticleInternational wound journal2016

Increased expression of endosomal members of toll-like receptor family abrogates wound healing in patients with type 2 diabetes mellitus.

Kanhaiya Singh, Neeraj K Agrawal, Sanjeev K Gupta, Gyanendra Mohan, Sunanda Chaturvedi, Kiran Singh

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International wound journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
7.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Epigenetic basis of diabetic vasculopathy.Frontiers in endocrinology · 2022
    Review
  6. Pattern recognition receptors in health and diseases.Signal transduction and targeted therapy · 2021
    Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Toll-like receptor 2 and type 2 diabetes.Cellular & molecular biology letters · 2016
    Review
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Kanhaiya SinghDepartment of Molecular & Human Genetics, Banaras Hindu University, Varanasi, 221005, India.
Neeraj K AgrawalDepartment of Endocrinology and Metabolism, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Sanjeev K GuptaDepartment of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Gyanendra MohanDepartment of Pathology, Indian Railway Cancer Hospital and Research Center, N.E.R., Varanasi, 221002, India.
Sunanda ChaturvediDepartment of Pathology, Indian Railway Cancer Hospital and Research Center, N.E.R., Varanasi, 221002, India.
Kiran SinghDepartment of Molecular & Human Genetics, Banaras Hindu University, Varanasi, 221005, India. skiran@bhu.ac.in.
Banaras Hindu University · INInstitute of Medical Sciences · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The inflammatory phase of wound healing cascade is an important determinant of the fate of the wound. Acute inflammation is necessary to initiate proper wound healing, while chronic inflammation abrogates wound healing. Different endosomal members of toll-like receptor (TLR) family initiate inflammatory signalling via a range of different inflammatory mediators such as interferons, internal tissue damaged-associated molecular patterns (DAMPs) and hyperactive effector T cells. Sustained signalling of TLR9 and TLR7 contributes to chronic inflammation by activating the plasmacytoid dendritic cells. Diabetic wounds are also characterised by sustained inflammatory phase. The objective of this study was to analyse the differential expression of endosomal TLRs in human diabetic wounds compared with control wounds. We analysed the differential expression of TLR7 and TLR9 both at transcriptional and translational levels in wounds of 84 patients with type 2 diabetes mellitus (T2DM) and 6 control subjects without diabetes using quantitative real-time polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. TLR7 and TLR9 were significantly up-regulated in wounds of the patients with T2DM compared with the controls and were dependent on the infection status of the diabetic wounds, and wounds with microbial infection exhibited lower expression levels of endosomal TLRs. Altered endosomal TLR expression in T2DM subjects might be associated with wound healing impairment.

Indexed as

AdultAgedCase-Control StudiesDiabetes Mellitus, Type 2Diabetic FootFemaleHumansMaleMiddle AgedRNA, MessengerToll-Like Receptor 7Toll-Like Receptor 9Wound HealingRNA, MessengerTLR7 protein, humanTLR9 protein, humanToll-Like Receptor 7Toll-Like Receptor 9Diabetic foot ulcerT2DMTLR7TLR9Toll-like receptorsWound healing impairment

Identifiers

PMID25586463
PMCPMC7949499
OpenAlexW1868200399

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.