ArticleG3 (Bethesda, Md.)2015
Rapid and inexpensive whole-genome genotyping-by-sequencing for crossover localization and fine-scale genetic mapping.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 147 citations in OpenAlex.
- SNI1/NSE5 is a cold-sensitive brake on class II crossovers in Arabidopsis.Nature plants · 2026Article
- High-resolution global recombination mapping in C. elegans reveals sexual dimorphisms shaped by meiotic chromosomal features and structures.PLoS genetics · 2026Article
- High-resolution global recombination mapping inbioRxiv : the preprint server for biology · 2026Article
- scGeno: a Hidden Markov Model approach to denoise chromosome-scale genotypes from single-cell data.Bioinformatics advances · 2026Article
- SCEP3 initiates synapsis and implements crossover interference in Arabidopsis.Nature plants · 2025Article
- COmapper: high-resolution mapping of meiotic crossovers by long-read sequencing in Arabidopsis.The New phytologist · 2025Article
- Maximizing meiotic crossover rates reveals the map of Crossover Potential.Nature communications · 2025Article
- Histone variant H2A.W7 represses meiotic crossover formation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Species-wide gene editing of a flowering regulator reveals hidden phenotypic variation.PLoS biology · 2025Article
- Plant sperm cell sequencing for genome phasing and determination of meiotic crossover points.Nature protocols · 2025Review
- Genetic dissection of MutL complexes in Arabidopsis meiosis.Nucleic acids research · 2025Article
- The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis.The Plant cell · 2024Article
- Modular control of vertebrate axis segmentation in time and space.The EMBO journal · 2024Article
- Enhanced recombination empowers the detection and mapping of Quantitative Trait Loci.Communications biology · 2024Article
- Meiotic recombination dynamics in plants with repeat-based holocentromeres shed light on the primary drivers of crossover patterning.Nature plants · 2024Article
- Control of meiotic crossover interference by a proteolytic chaperone network.Nature plants · 2024Article
- MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism.Nature communications · 2023Article
- Article
- The regulation of meiotic crossover distribution: a coarse solution to a century-old mystery?Biochemical Society transactions · 2023Review
- HEIP1 is required for efficient meiotic crossover implementation and is conserved from plants to humans.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reshuffling of existing genetic variation during meiosis is important both during evolution and in breeding. The reassortment of genetic variants relies on the formation of crossovers (COs) between homologous chromosomes. The pattern of genome-wide CO distributions can be rapidly and precisely established by the short-read sequencing of individuals from F2 populations, which in turn are useful for quantitative trait locus (QTL) mapping. Although sequencing costs have decreased precipitously in recent years, the costs of library preparation for hundreds of individuals have remained high. To enable rapid and inexpensive CO detection and QTL mapping using low-coverage whole-genome sequencing of large mapping populations, we have developed a new method for library preparation along with Trained Individual GenomE Reconstruction, a probabilistic method for genotype and CO predictions for recombinant individuals. In an example case with hundreds of F2 individuals from two Arabidopsis thaliana accessions, we resolved most CO breakpoints to within 2 kb and reduced a major flowering time QTL to a 9-kb interval. In addition, an extended region of unusually low recombination revealed a 1.8-Mb inversion polymorphism on the long arm of chromosome 4. We observed no significant differences in the frequency and distribution of COs between F2 individuals with and without a functional copy of the DNA helicase gene RECQ4A. In summary, we present a new, cost-efficient method for large-scale, high-precision genotyping-by-sequencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.