ArticlePloS one2015
Primary isolation strain determines both phage type and receptors recognised by Campylobacter jejuni bacteriophages.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 68 citations in OpenAlex.
- Regulatory Landscape and the Potential of Bacteriophage Applications in the United States' Food Industry.Journal of food protection · 2025Review
- An insight into the applications of bacteriophages against food-borne pathogens.Journal of food science and technology · 2025Review
- Campycins are novel broad-spectrum antibacterials killing Campylobacter jejuni.Applied microbiology and biotechnology · 2024Article
- Investigating bacteriophages as a novel multiple-hurdle measure against Campylobacter: field trials in commercial broiler plants.Scientific reports · 2024Article
- A hybrid receptor binding protein enables phage F341 infection ofFrontiers in microbiology · 2024Article
- Motility inMicroorganisms · 2023Article
- Bacteriophage cocktail application for Campylobacter mitigation - from in vitro to in vivo.BMC microbiology · 2023Article
- Bacteriophages and food safety: An updated overview.Food science & nutrition · 2023Review
- Evaluation of core genome and whole genome multilocus sequence typing schemes forMicrobial genomics · 2023Article
- Increased sensitivity of Aggregatibacter actinomycetemcomitans to human serum is mediated by induction of a bacteriophage.Molecular oral microbiology · 2023Article
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- Application of Bacteriophages to LimitFrontiers in microbiology · 2021Review
- Isolation and Characterization of Group IIIFrontiers in microbiology · 2021Article
- Developing Innolysins AgainstFrontiers in microbiology · 2021Article
- Phage Biocontrol of Campylobacter: A One Health Approach.Current topics in microbiology and immunology · 2021Review
- Natural Competence and Horizontal Gene Transfer in Campylobacter.Current topics in microbiology and immunology · 2021Article
- Identification of Novel Phage Resistance Mechanisms inFrontiers in microbiology · 2021Article
- Two Distinct Modes of Lysis Regulation inViruses · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we isolated novel bacteriophages, infecting the zoonotic bacterium Campylobacter jejuni. These phages may be used in phage therapy of C. jejuni colonized poultry to prevent spreading of the bacteria to meat products causing disease in humans. Many C. jejuni phages have been isolated using NCTC12662 as the indicator strain, which may have biased the selection of phages. A large group of C. jejuni phages rely on the highly diverse capsular polysaccharide (CPS) for infection and recent work identified the O-methyl phosphoramidate modification (MeOPN) of CPS as a phage receptor. We therefore chose seven C. jejuni strains each expressing different CPS structures as indicator strains in a large screening for phages in samples collected from free-range poultry farms. Forty-three phages were isolated using C. jejuni NCTC12658, NCTC12662 and RM1221 as host strains and 20 distinct phages were identified based on host range analysis and genome restriction profiles. Most phages were isolated using C. jejuni strains NCTC12662 and RM1221 and interestingly phage genome size (140 kb vs. 190 kb), host range and morphological appearance correlated with the isolation strain. Thus, according to C. jejuni phage grouping, NCTC12662 and NCTC12658 selected for CP81-type phages, while RM1221 selected for CP220-type phages. Furthermore, using acapsular ∆kpsM mutants we demonstrated that phages isolated on NCTC12658 and NCTC12662 were dependent on the capsule for infection. In contrast, CP220-type phages isolated on RM1221 were unable to infect non-motile ∆motA mutants, hence requiring motility for successful infection. Hence, the primary phage isolation strain determines both phage type (CP81 or CP220) as well as receptors (CPS or flagella) recognised by the isolated phages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.