ArticleMolecular therapy. Nucleic acids2015
Preparation of Small RNAs Using Rolling Circle Transcription and Site-Specific RNA Disconnection.
Article in Molecular therapy. Nucleic acids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- An Apple a Day? The Hypothesis of Cross-Kingdom Gene Regulation by Plant miRNAs in Mammals and Its Controversies.International journal of molecular sciences · 2026Review
- Entropy-drive DNA circuit coupled with no-promoter rolling circle transcription to synthesize fluorescent gold nanoclusters for label-free detection of breast cancer biomarkers.Mikrochimica acta · 2026Article
- Therapeutic applications of RNA nanostructures.RSC advances · 2024Review
- LINCATRA: Two-cycle method to amplify RNA for transcriptome analysis from formalin-fixed paraffin-embedded tissue.Heliyon · 2024Article
- PACRAT: pathogen detection with aptamer-observed cascaded recombinase polymerase amplification-in vitro transcription.RNA (New York, N.Y.) · 2024Article
- Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR.Molecular therapy. Nucleic acids · 2023Article
- One-Pot Production of RNA in High Yield and Purity Through Cleaving Tandem Transcripts.Molecules (Basel, Switzerland) · 2020Article
- SSD - a free software for designing multimeric mono-, bi- and trivalent shRNAs.Genetics and molecular biology · 2020Article
- Article
- Cyclization of secondarily structured oligonucleotides to single-stranded rings by usingRSC advances · 2018Article
- Extensive profiling of circular RNAs and the potential regulatory role of circRNA-000284 in cell proliferation and invasion of cervical cancer via sponging miR-506.American journal of translational research · 2018Article
- Highly efficient preparation of single-stranded DNA rings by T4 ligase at abnormally low Mg(II) concentration.Nucleic acids research · 2017Article
- Digestion of Nucleic Acids Starts in the Stomach.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A facile and robust RNA preparation protocol was developed by combining rolling circle transcription (RCT) with RNA cleavage by RNase H. Circular DNA with a complementary sequence was used as the template for promoter-free transcription. With the aid of a 2'-O-methylated DNA, the RCT-generated tandem repeats of the desired RNA sequence were disconnected at the exact end-to-end position to harvest the desired RNA oligomers. Compared with the template DNA, more than 4 × 10(3) times the amount of small RNA products were obtained when modest cleavage was carried out during transcription. Large amounts of RNA oligomers could easily be obtained by simply increasing the reaction volume.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.