ArticleInternational journal of molecular sciences2015
Rapid and sensitive identification of the herbal tea ingredient Taraxacum formosanum using loop-mediated isothermal amplification.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Loop-mediated isothermal amplification (LAMP) for quality control of Coscinium fenestratum: a colorimetric platform for detecting Anamirta cocculus adulteration.Protoplasma · 2026Article
- Shedding light on deception: LAMP as a tool for detecting adulterants in herbal medicine.Protoplasma · 2026Review
- Single nucleotide polymorphism-based visual identification ofFrontiers in plant science · 2024Article
- Potential for the development ofFrontiers in immunology · 2024Article
- Development of Loop-Mediated Isothermal Amplification Method for Rapid and Sensitive Identification ofMethods and protocols · 2023Article
- Genetic Authentication of the Medicinal PlantInternational journal of molecular sciences · 2023Article
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Horizon scan of DNA-based methods for quality control and monitoring of herbal preparations.Frontiers in pharmacology · 2023Review
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- Specific, sensitive and rapidSaudi journal of biological sciences · 2021Article
- Specific and rapid identification of theBioscience reports · 2019Article
- Molecular authentication ofSaudi journal of biological sciences · 2019Article
- The development of loop-mediated isothermal amplification (LAMP) assays for the rapid authentication of five forbidden vegetables in strict vegetarian diets.Scientific reports · 2017Article
- Poisonous or non-poisonous plants? DNA-based tools and applications for accurate identification.International journal of legal medicine · 2017Review
- Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification.Frontiers in plant science · 2016Article
- Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification.Frontiers in plant science · 2016Review
- Authentication of medicinal plants by DNA markers.Plant gene · 2015Review
- DNA Barcoding and Pharmacovigilance of Herbal Medicines.Drug safety · 2015Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDNA) of TF. LAMP amplicons were successfully amplified and detected when purified genomic DNA of TF was added in the LAMP reaction under isothermal condition (65 °C) within 45 min. These specific LAMP primers have high specificity and can accurately discriminate Taraxacum formosanum from other adulterant plants; 1 pg of genomic DNA was determined to be the detection limit of the LAMP assay. In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control.
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