ArticleJournal of the American Chemical Society2015
Fluorescent flippers for mechanosensitive membrane probes.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
63 citing papers in PubMed.
- Neuroligin-3-CSPG4 interaction maintains oligodendrocyte precursor cell progenitor state and promotes glioma proliferation through mechanotransduction.Nature neuroscience · 2026Article
- TiFM2.0 - versatile mechanical measurement and actuation in live embryos.Development (Cambridge, England) · 2026Article
- Review
- Mechanisms of active wetting and fluidification in epithelial cell collectives.Nature materials · 2026Article
- Modeling Targeted Mechanochemistry in Polymeric Solids.Chemical reviews · 2026Review
- Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.bioRxiv : the preprint server for biology · 2026Article
- Lysosomal lipid metabolism promotes tumor cell invasion through local energetics and membrane lipid remodeling.bioRxiv : the preprint server for biology · 2026Article
- Adherent cells sustain membrane tension gradients independently of migration.Nature communications · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Lipid-Directed Covalent Labeling of Plasma Membranes for Long-Term Imaging, Barcoding and Manipulation of Cells.JACS Au · 2025Article
- Advancing the Mechanosensitivity of Atropisomeric Diarylethene Mechanophores through a Lever-Arm Effect.Journal of the American Chemical Society · 2025Article
- Redefining Molecular Probes for Monitoring Subcellular Environment: A Perspective.Analytical chemistry · 2024Review
- Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes.Nature protocols · 2024Review
- Tensing Flipper: Photosensitized Manipulation of Membrane Tension, Lipid Phase Separation, and Raft Protein Sorting in Biological Membranes.Journal of the American Chemical Society · 2024Article
- Fluorescence lifetime imaging microscopy of flexible and rigid dyes probes the biophysical properties of synthetic and biological membranes.Biophysical journal · 2024Article
- The Plasma Membrane and Mechanoregulation in Cells.ACS omega · 2024Review
- Methods for making and observing model lipid droplets.Cell reports methods · 2024Article
- Research progress on the regulatory role of cell membrane surface tension in cell behavior.Heliyon · 2024Review
- Molecular Probing of the Microscopic Pressure at Contact Interfaces.Journal of the American Chemical Society · 2024Article
- Observing mechanosensitive channels in action in living bacteria.Biophysical reports · 2024Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report, "fluorescent flippers" are introduced to create planarizable push-pull probes with the mechanosensitivity and fluorescence lifetime needed for practical use in biology. Twisted push-pull scaffolds with large and bright dithienothiophenes and their S,S-dioxides as the first "fluorescent flippers" are shown to report on the lateral organization of lipid bilayers with quantum yields above 80% and lifetimes above 4 ns. Their planarization in liquid-ordered (Lo) and solid-ordered (So) membranes results in red shifts in excitation of up to +80 nm that can be transcribed into red shifts in emission of up to +140 nm by Förster resonance energy transfer (FRET). These unique properties are compatible with multidomain imaging in giant unilamellar vesicles (GUVs) and cells by confocal laser scanning or fluorescence lifetime imaging microscopy. Controls indicate that strong push-pull macrodipoles are important, operational probes do not relocate in response to lateral membrane reorganization, and two flippers are indeed needed to "really swim," i.e., achieve high mechanosensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.