ArticleJournal of clinical and diagnostic research : JCDR2014
Comparison of various methods used to detect biofilm production of Candida species.
Article in Journal of clinical and diagnostic research : JCDR, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- CApEsid biOfilm: a suggested pipeline for clinical phage microbiology for biofilm infections based on comparative method study.Scientific reports · 2026Article
- Relationship Between Virulence Factor Activities, Cytotoxicity ofDentistry journal · 2025Article
- Inhibitory Efficacy ofBiology · 2025Article
- A trans-acting sRNA SaaS targeting hilD, cheA and csgA to inhibit biofilm formation of S. Enteritidis.Journal of advanced research · 2025Article
- SUMO-targeted Ubiquitin Ligases as crucial mediators of protein homeostasis in Candida glabrata.PLoS pathogens · 2024Article
- Inhibition ofAntibiotics (Basel, Switzerland) · 2024Article
- Supramolecular Self-Associating Amphiphiles Inhibit Biofilm Formation by the Critical Pathogens,ACS omega · 2024Article
- Characterization of chicken eggs associatedAnimal biotechnology · 2023Article
- Biofilm formation and its effect on the management of culture-positive bacterial endophthalmitis.Indian journal of ophthalmology · 2022Article
- Review
- Evaluation of the biofilm life cycle betweenFrontiers in cellular and infection microbiology · 2022Article
- Biofilm-Forming Potential of Ocular FluidMicroorganisms · 2021Article
- Nanovectorized Microalgal Extracts to FightAntibiotics (Basel, Switzerland) · 2020Article
- The antifungal agent of silver nanoparticles activated by diode laser as light source to reduce C. albicans biofilms: an in vitro study.Lasers in medical science · 2019Article
- Biofilm Specific Activity: A Measure to Quantify Microbial Biofilm.Microorganisms · 2019Article
- Antifungal activity of magnoflorine against Candida strains.World journal of microbiology & biotechnology · 2018Article
- Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action.Virulence · 2018Review
- ThemSphereArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe biofilm of organisms can be considered as a virulence factor because of the resistance towards antimicrobial agents. Formation of Candida biofilms are observed in blood, mucosal surface and most medical devices i.e. nonliving objects in patient's body. The study was undertaken to conclude the most sensitive and specific test for detection of biofilm formation. MATERIALS AND
methodsQuantitative measurement of biofilm formation was assessed by three methods- 1) XTT reductionassay, 2) Percentage Transmission (%T) and 3) Crystal Violet assay in microtitre plate for 425 Candida isolates.
resultsOut of 425 Candida strains, XTT reduction assay gave 72 Biofilm positive strains accounting for 16.94 %. The percentage transmission (% T) method gave 56 strains Biofilm positive (13.17 %) and Crystal violet assay gave 50 positive strains (11.76 %). Compared to Percentage Transmission (% T) and Crystal violet method, XTT reduction assay gave maximum percentage of Biofilm positivity.
conclusionIn comparison of 3 methods used for detection of biofilm formation, XTT reduction assay was the most sensitive and specific method.
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