ArticleJournal of bacteriology2015
Slow leakage of Ca-dipicolinic acid from individual bacillus spores during initiation of spore germination.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- A mathematical kinetic model of memory iniScience · 2026Article
- Early activation of bioenergetic metabolism powers bacterial spore germination.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bacillus subtilis as cell factory for enhanced production of the biopolymer precursor pyridine-2,6-dicarboxylic acid.Microbial cell factories · 2025Article
- Characterization of individual spores of two biological insecticides, Bacillus thuringiensis and Lysinibacillus sphaericus, in response to glutaraldehyde using single-cell optical approaches.Archives of microbiology · 2024Article
- Electrochemical potential enables dormant spores to integrate environmental signals.Science (New York, N.Y.) · 2022Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- Heat Activation and Inactivation of Bacterial Spores: Is There an Overlap?Applied and environmental microbiology · 2022Article
- Predicting the Structure and Dynamics of Membrane Protein GerAB fromInternational journal of molecular sciences · 2021Article
- Mode of Action of Disinfection Chemicals on the Bacterial Spore Structure and Their Raman Spectra.Analytical chemistry · 2021Article
- Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation.Frontiers in microbiology · 2021Review
- Bioluminescence dynamics in single germinating bacterial spores reveal metabolic heterogeneity.Journal of the Royal Society, Interface · 2020Article
- A luminous off-on probe for the determination of 2,6-pyridinedicarboxylic acid as an anthrax biomarker based on water-soluble cadmium sulfide quantum dots.Mikrochimica acta · 2020Article
- Single-cell analysis reveals individual spore responses to simulated space vacuum.NPJ microgravity · 2018Article
- Analysis of the Germination of IndividualFrontiers in microbiology · 2017Article
- Use of Raman Spectroscopy and Phase-Contrast Microscopy To Characterize Cold Atmospheric Plasma Inactivation of Individual Bacterial Spores.Applied and environmental microbiology · 2016Article
- Article
- Raman Spectroscopy of Optically Trapped Single Biological Micro-Particles.Sensors (Basel, Switzerland) · 2015Review
- Characterization of the Dynamic Germination of Individual Clostridium difficile Spores Using Raman Spectroscopy and Differential Interference Contrast Microscopy.Journal of bacteriology · 2015Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When exposed to nutrient or nonnutrient germinants, individual Bacillus spores can return to life through germination followed by outgrowth. Laser tweezers, Raman spectroscopy, and either differential interference contrast or phase-contrast microscopy were used to analyze the slow dipicolinic acid (DPA) leakage (normally ∼20% of spore DPA) from individual spores that takes place prior to the lag time, Tlag, when spores begin rapid release of remaining DPA. Major conclusions from this work with Bacillus subtilis spores were as follows: (i) slow DPA leakage from wild-type spores germinating with nutrients did not begin immediately after nutrient exposure but only at a later heterogeneous time T1; (ii) the period of slow DPA leakage (ΔTleakage = Tlag - T1) was heterogeneous among individual spores, although the amount of DPA released in this period was relatively constant; (iii) increases in germination temperature significantly decreased T1 times but increased values of ΔTleakage; (iv) upon germination with l-valine for 10 min followed by addition of d-alanine to block further germination, all germinated spores had T1 times of less than 10 min, suggesting that T1 is the time when spores become committed to germinate; (v) elevated levels of SpoVA proteins involved in DPA movement in spore germination decreased T1 and Tlag times but not the amount of DPA released in ΔTleakage; (vi) lack of the cortex-lytic enzyme CwlJ increased DPA leakage during germination due to longer ΔTleakage times in which more DPA was released; and (vii) there was slow DPA leakage early in germination of B. subtilis spores by the nonnutrients CaDPA and dodecylamine and in nutrient germination of Bacillus cereus and Bacillus megaterium spores. Overall, these findings have identified and characterized a new early event in Bacillus spore germination.
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