ArticlePlant physiology2015
Reexamination of chlorophyllase function implies its involvement in defense against chewing herbivores.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 108 citations in OpenAlex.
- Metabolism and regulation of chlorophyll and carotenoids in tomato fruits.Horticulture research · 2026Article
- Effects of Light on Faecal Case-bearing Larvae of the Leaf Beetle Chlamisus laticollis (Coleoptera: Chrysomelidae: Cryptocephalinae) Accumulating Phototoxic Chlorophyll Catabolites.Journal of chemical ecology · 2026Article
- Phytotoxicity and metal mobility in soils contaminated with mine tailings.Environmental geochemistry and health · 2026Article
- Cucumber Green Mottle Mosaic Virus Decreases ChlorophyllPlants (Basel, Switzerland) · 2025Article
- Chlorophyllase (PsCLH1) and light-harvesting chlorophyll a/b binding protein 1 (PsLhcb1) and PsLhcb5 maintain petal greenness in Paeonia suffruticosa 'Lv Mu Yin Yu'.Journal of advanced research · 2025Article
- A family of α/β hydrolases removes phytol from chlorophyll metabolites for tocopherol biosynthesis in Arabidopsis.The Plant cell · 2025Article
- Comparative metabolites analysis of resistant, susceptible and wild rice species in response to bacterial blight disease.BMC plant biology · 2025Article
- Chlorophyllase fromACS bio & med chem Au · 2024Article
- Arabidopsis Transcriptomics Reveals the Role of Lipoxygenase2 (AtLOX2) in Wound-Induced Responses.International journal of molecular sciences · 2024Article
- Genome-wide association study of agronomic traits related to nitrogen use efficiency in Henan wheat.BMC genomics · 2024Article
- A structure-function analysis of chlorophyllase reveals a mechanism for activity regulation dependent on disulfide bonds.The Journal of biological chemistry · 2023Article
- Article
- Genes, Structural, and Biochemical Characterization of Four Chlorophyllases fromInternational journal of molecular sciences · 2022Article
- Genome-wide association identifies a missing hydrolase for tocopherol synthesis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Multiomics Approach To Decipher the Origin of Chlorophyll Content in Virgin Olive Oil.Journal of agricultural and food chemistry · 2022Article
- Evolution of chlorophyll degradation is associated with plant transition to land.The Plant journal : for cell and molecular biology · 2022Article
- Transcriptome Analysis Revealed a Positive Role of Ethephon on Chlorophyll Metabolism ofPlants (Basel, Switzerland) · 2022Article
- Plant growth promoting bacteria (PGPR) induce antioxidant tolerance against salinity stress through biochemical and physiological mechanisms.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Bulk segregant analysis-sequencing and RNA-Seq analyses reveal candidate genes associated with albino phenotype inFrontiers in plant science · 2022Article
- Evolutionary genomics and biosynthetic potential of novel environmental Actinobacteria.Applied microbiology and biotechnology · 2021Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlorophyllase (CLH) is a common plant enzyme that catalyzes the hydrolysis of chlorophyll to form chlorophyllide, a more hydrophilic derivative. For more than a century, the biological role of CLH has been controversial, although this enzyme has been often considered to catalyze chlorophyll catabolism during stress-induced chlorophyll breakdown. In this study, we found that the absence of CLH does not affect chlorophyll breakdown in intact leaf tissue in the absence or the presence of methyl-jasmonate, which is known to enhance stress-induced chlorophyll breakdown. Fractionation of cellular membranes shows that Arabidopsis (Arabidopsis thaliana) CLH is located in the endoplasmic reticulum and the tonoplast of intact plant cells. These results indicate that CLH is not involved in endogenous chlorophyll catabolism. Instead, we found that CLH promotes chlorophyllide formation upon disruption of leaf cells, or when it is artificially mistargeted to the chloroplast. These results indicate that CLH is responsible for chlorophyllide formation after the collapse of cells, which led us to hypothesize that chlorophyllide formation might be a process of defense against chewing herbivores. We found that Arabidopsis leaves with genetically enhanced CLH activity exhibit toxicity when fed to Spodoptera litura larvae, an insect herbivore. In addition, purified chlorophyllide partially suppresses the growth of the larvae. Taken together, these results support the presence of a unique binary defense system against insect herbivores involving chlorophyll and CLH. Potential mechanisms of chlorophyllide action for defense are discussed.
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