ArticleScientific reports2015
Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Nanomaterials as plant delivery carriers: transport mechanisms and design principles.Science China. Life sciences · 2026Review
- Nanodelivery of nucleic acids for plant genetic engineering.Discover nano · 2025Review
- High Aspect Ratio Polymer Nanocarriers for Gene Delivery and Expression in Plants.Nano letters · 2025Article
- Development of a Versatile Plant-Derived Mitochondrial Targeting Sequence Based on a Reporter Protein Sorting Analysis and Biological Information.ACS chemical biology · 2024Article
- Polymeric Nanocarriers Autonomously Cross the Plant Cell Wall and Enable Protein Delivery for Stress Sensing.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Nature-inspired peptide of MtDef4 C-terminus tail enables protein delivery in mammalian cells.Scientific reports · 2024Article
- Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells.International journal of molecular sciences · 2023Review
- Plant Mitochondrial-Targeted Gene Delivery by Peptide/DNA Micelles Quantitatively Surface-Modified with Mitochondrial Targeting and Membrane-Penetrating Peptides.Biomacromolecules · 2023Article
- Cell-Penetrating Peptides for Use in Development of Transgenic Plants.Molecules (Basel, Switzerland) · 2023Review
- Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay.BMC research notes · 2023Article
- Mitochondrial gene defects in Arabidopsis can broadly affect mitochondrial gene expression through copy number.Plant physiology · 2023Article
- Regulation and safety measures for nanotechnology-based agri-products.Frontiers in genome editing · 2023Review
- Relaxation of the Plant Cell Wall Barrier via Zwitterionic Liquid Pretreatment for Micelle-Complex-Mediated DNA Delivery to Specific Plant Organelles.Angewandte Chemie (International ed. in English) · 2022Article
- Mitochondria targeting drugs for neurodegenerative diseases-Design, mechanism and application.Acta pharmaceutica Sinica. B · 2022Review
- Non-transgenic Gene ModulationACS nano · 2022Article
- Molecular dynamics study of the internalization of cell-penetrating peptides containing unnatural amino acids across membranes.Nanoscale advances · 2022Article
- Applications of CPPs in Genome Editing of Plants.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Cell-Penetrating Peptides.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf.Frontiers in plant science · 2022Article
- Effects of mitochondria-selective fluorescent probes on mitochondrial movement inPlant biotechnology (Tokyo, Japan) · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Available methods in plant genetic transformation are nuclear and plastid transformations because similar procedures have not yet been established for the mitochondria. The double membrane and small size of the organelle, in addition to its large population in cells, are major obstacles in mitochondrial transfection. Here we report the intracellular delivery of exogenous DNA localized to the mitochondria of Arabidopsis thaliana using a combination of mitochondria-targeting peptide and cell-penetrating peptide. Low concentrations of peptides were sufficient to deliver DNA into the mitochondria and expression of imported DNA reached detectable levels within a short incubation period (12 h). We found that electrostatic interaction with the cell membrane is not a critical factor for complex internalization, instead, improved intracellular penetration of mitochondria-targeted complexes significantly enhanced gene transfer efficiency. Our results delineate a simple and effective peptide-based method, as a starting point for the development of more sophisticated plant mitochondrial transfection strategies.
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