ArticleNature communications2015
Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Engineering Decellularized Extracellular Matrix-Incorporated Apical-Out Airway Organoids.Bio-protocol · 2026Article
- Recapitulating apicobasal tissue polarity in extracellular matrix-incorporated airway organoids.Biomaterials · 2026Article
- Article
- PI3Kδ activity controls plasticity and discriminates between EMT and stemness based on distinct TGFβ signaling.Communications biology · 2022Article
- PI(4,5)P2 controls slit diaphragm formation and endocytosis in Drosophila nephrocytes.Cellular and molecular life sciences : CMLS · 2022Article
- Rab22a regulates the establishment of epithelial polarity.Small GTPases · 2021Article
- Development of a functional salivary gland tissue chip with potential for high-content drug screening.Communications biology · 2021Article
- Phospholipids of the Plasma Membrane - Regulators or Consequence of Cell Polarity?Frontiers in cell and developmental biology · 2020Review
- Article
- The role of polarisation of circulating tumour cells in cancer metastasis.Cellular and molecular life sciences : CMLS · 2019Review
- Mesenchymal-epithelial transition in development and reprogramming.Nature cell biology · 2019Review
- The phospholipid PI(3,4)PNature communications · 2018Article
- Laminins in Epithelial Cell Polarization: Old Questions in Search of New Answers.Cold Spring Harbor perspectives in biology · 2017Review
- Clinical spectrum and features of activated phosphoinositide 3-kinase δ syndrome: A large patient cohort study.The Journal of allergy and clinical immunology · 2017Article
- PI3K/SHIP2/PTEN pathway in cell polarity and hepatitis C virus pathogenesis.World journal of hepatology · 2017Review
- GSK-3β and MMP-9 Cooperate in the Control of Dendritic Spine Morphology.Molecular neurobiology · 2017Article
- Synthetic hydrogels mimicking basement membrane matrices to promote cell-matrix interactions.Matrix biology : journal of the International Society for Matrix Biology · 2017Review
- Rab14 specifies the apical membrane through Arf6-mediated regulation of lipid domains and Cdc42.Scientific reports · 2016Article
- Phosphorylation-dependent Akt-Inversin interaction at the basal body of primary cilia.The EMBO journal · 2016Article
- Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening.Nature communications · 2016Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
Signalling triggered by adhesion to the extracellular matrix plays a key role in the spatial orientation of epithelial polarity and formation of lumens in glandular tissues. Phosphoinositide 3-kinase signalling in particular is known to influence the polarization process during epithelial cell morphogenesis. Here, using Madin-Darby canine kidney epithelial cells grown in 3D culture, we show that the p110δ isoform of phosphoinositide 3-kinase co-localizes with focal adhesion proteins at the basal surface of polarized cells. Pharmacological, siRNA- or kinase-dead-mediated inhibition of p110δ impair the early stages of lumen formation, resulting in inverted polarized cysts, with no laminin or type IV collagen assembly at cell/extracellular matrix contacts. p110δ also regulates the organization of focal adhesions and membrane localization of dystroglycan. Thus, we uncover a previously unrecognized role for p110δ in epithelial cells in the orientation of the apico-basal axis and lumen formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.