ArticleParasites & vectors2015
Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Review
- Administration of Trichinella spiralis Antigens Alleviated Diabetic Nephropathy in Diabetic Mice.Acta parasitologica · 2025Article
- Proteome identification of common immunological proteins of two nematode parasites.Parasites, hosts and diseases · 2024Article
- AdvancingPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis.Veterinary research · 2024Article
- Trichinella spiralis -induced immunomodulation signatures on gut microbiota and metabolic pathways in mice.PLoS pathogens · 2024Article
- Article
- Nano magnetic-based ELISA and nano magnetic-based latex agglutination test for diagnosis of experimental trichinellosis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Roles of Cysteine Proteases in Biology and Pathogenesis of Parasites.Microorganisms · 2023Article
- Metabolite profiling ofFrontiers in cellular and infection microbiology · 2023Article
- Article
- Identification and profiling of Trichinella spiralis circulating antigens and proteins in sera of mice with trichinellosis.PloS one · 2022Article
- Prospects of Using High-Throughput Proteomics to Underpin the Discovery of Animal Host-Nematode Interactions.Pathogens (Basel, Switzerland) · 2021Review
- Development of a rapid and sensitive immunochromatographic strip based on EuNPs-ES fluorescent probe for the detection of early Trichinella spiralis-specific IgG antibody in pigs.Veterinary research · 2021Article
- Trichuris trichiura egg extract proteome reveals potential diagnostic targets and immunomodulators.PLoS neglected tropical diseases · 2021Article
- The intestinal milieu influences the immunoproteome of male and femaleParasitology · 2020Article
- Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe.Foods (Basel, Switzerland) · 2020Review
- Immunization with a Recombinant Protein ofVaccines · 2020Article
- The cysteine protease ATG4B of Trichinella spiralis promotes larval invasion into the intestine of the host.Veterinary research · 2020Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundTrichinellosis, a widespread zoonosis, is regarded as an emerging or reemerging disease. Effective treatment and prognosis of trichinellosis depends on early diagnosis of the infection. The objective of this study was to identify sensitive and specific antigens for early diagnosis or effective vaccine antigens for preventing infection.
methodsThe somatic proteins of T. spiralis adult worms were separated by two-dimensional gel electrophoresis (2-DE). The separated proteins were probed with early infection sera from swine or mice infected with T. spiralis for 7 days. The primary immunoreactive spots were characterized by MALDI-TOF/TOF-MS analysis in combination with bioinformatics. The identified proteins were annotated using WEGO based on their functions. The immunodominant protein was chosen for expression as recombinant protein in E. coli and the purified recombinant protein was used to confirm its antigenicity by Western blot with the same infection sera.
resultsApproximately 300 spots were separated by 2-DE, with molecular weights ranging from 10 to 130 kDa, and pI values ranging from pH 4 to 10. The sera from swine and mice infected with T. spiralis for 7 days recognized 64 proteins. MALDI-TOF/TOF-MS analysis identified 55 proteins, some with different isoforms. Finally, 40 individual immunoreactive proteins were obtained with a wide range of biological functions. Several proteins, such as heat shock protein 70, 14-3-3 protein, and cysteine protease could be used as immunodiagnostic or vaccine antigens. Among these identified proteins, the highly immunodominant Ts14-3-3 was chosen for expression in E. coli and purified recombinant Ts14-3-3 was able to be strongly recognized by the same T. spiralis infected sera used for identifying these antigens, therefore the most promising antigen for early immunodiagnosis of Trichinella infection.
conclusionsA total of 64 proteins from the adult worm were recognized by early infection sera from swine and mice infected with T. spiralis for 7 days. Several proteins, are of particular interest as immunodiagnostic or vaccine antigens, especially with Ts14-3-3 as most promising due to its highly immunogenicity during early infection, expressed protein can be recognized by Trichinella early infection sera and the native Ts14-3-3 expression in both adult and larval stages.
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