ArticleMolecular microbiology2015
Transcriptome analysis of cyclic AMP-dependent protein kinase A-regulated genes reveals the production of the novel natural compound fumipyrrole by Aspergillus fumigatus.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
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- Fungal BGCs for Production of Secondary Metabolites: Main Types, Central Roles in Strain Improvement, and Regulation According to the Piano Principle.International journal of molecular sciences · 2023Review
- Regulation of pseurotin A biosynthesis by GliZ and zinc in Aspergillus fumigatus.Scientific reports · 2023Article
- Modular Inducible Multigene Expression System for Filamentous Fungi.Microbiology spectrum · 2022Article
- Role of Dicer-Dependent RNA Interference in Regulating Mycoparasitic Interactions.Microbiology spectrum · 2021Article
- Presence, Mode of Action, and Application of Pathway Specific Transcription Factors inInternational journal of molecular sciences · 2021Review
- Determining Aspergillus fumigatus transcription factor expression and function during invasion of the mammalian lung.PLoS pathogens · 2021Article
- Genome-based engineering of ligninolytic enzymes in fungi.Microbial cell factories · 2021Review
- Natural products in the predatory defence of the filamentous fungal pathogenBeilstein journal of organic chemistry · 2021Review
- Controlled Transcription of Regulator GeneMicroorganisms · 2020Article
- Exposure ofJournal of fungi (Basel, Switzerland) · 2020Article
- Trichophyton rubrum Azole Resistance Mediated by a New ABC Transporter, TruMDR3.Antimicrobial agents and chemotherapy · 2019Article
- Recent advances in the genome mining ofMedChemComm · 2019Review
- A call to arms: Mustering secondary metabolites for success and survival of an opportunistic pathogen.PLoS pathogens · 2019Article
- The Lysine Deacetylase RpdA Is Essential for Virulence inFrontiers in microbiology · 2019Article
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Animal Models of Aspergillosis.Comparative medicine · 2018Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Aspergillus fumigatus is an opportunistic human pathogenic fungus causing life-threatening infections in immunocompromised patients. Adaptation to different habitats and also virulence of the fungus depends on signal perception and transduction by modules such as the cyclic AMP-dependent protein kinase A (PKA) pathway. Here, by transcriptome analysis, 632 differentially regulated genes of this important signaling cascade were identified, including 23 putative transcriptional regulators. The highest upregulated transcription factor gene was located in a previously unknown secondary metabolite gene cluster, which we named fmp, encoding an incomplete non-ribosomal peptide synthetase, FmpE. Overexpression of the regulatory gene fmpR using the Tet(On) system led to the specific expression of the other six genes of the fmp cluster. Metabolic profiling of wild type and fmpR overexpressing strain by HPLC-DAD and HPLC-HRESI-MS and structure elucidation by NMR led to identification of 5-benzyl-1H-pyrrole-2-carboxylic acid, which we named fumipyrrole. Fumipyrrole was not described as natural product yet. Chemical synthesis of fumipyrrole confirmed its structure. Interestingly, deletion of fmpR or fmpE led to reduced growth and sporulation of the mutant strains. Although fmp cluster genes were transcribed in infected mouse lungs, deletion of fmpR resulted in wild-type virulence in a murine infection model.
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