ArticleMolecular and cellular biology2015
Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 54 citations in OpenAlex.
- Replication and hematological characterization of human platelet reactivity genetic associations in men from the Caerphilly Prospective Study (CaPS).Journal of thrombosis and thrombolysis · 2016Trial
- Jade1 and the HBO1 histone acetyltransferase complex are spatial-selective cofactors of the pluripotency transcription factor Oct4.The Journal of biological chemistry · 2025Article
- Oct4 redox sensitivity potentiates reprogramming and differentiation.Genes & development · 2024Article
- RENGE infers gene regulatory networks using time-series single-cell RNA-seq data with CRISPR perturbations.Communications biology · 2023Article
- Oct1 cooperates with the Smad family of transcription factors to promote mesodermal lineage specification.Science signaling · 2023Article
- The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3.Autophagy · 2022Article
- MethReg: estimating the regulatory potential of DNA methylation in gene transcription.Nucleic acids research · 2022Article
- Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022Review
- 3D chromatin architecture and epigenetic regulation in cancer stem cells.Protein & cell · 2021Review
- A mathematical model exhibiting the effect of DNA methylation on the stability boundary in cell-fate networks.Epigenetics · 2021Article
- Crucial Functions of the JMJD1/KDM3 Epigenetic Regulators in Cancer.Molecular cancer research : MCR · 2021Review
- The role of FACT in managing chromatin: disruption, assembly, or repair?Nucleic acids research · 2020Review
- Polymorphisms inInternational journal of environmental research and public health · 2020Article
- Review
- Heterochromatin loosening by the Oct4 linker region facilitates Klf4 binding and iPSC reprogramming.The EMBO journal · 2020Article
- Identification of Transcription Factor/Gene Axis in Colon Cancer Using a Methylome Approach.Frontiers in genetics · 2020Article
- The role of α-ketoglutarate-dependent proteins in pluripotency acquisition and maintenance.The Journal of biological chemistry · 2019Review
- Article
- Chaperones and Beyond as Key Players in Pluripotency Maintenance.Frontiers in cell and developmental biology · 2019Review
- FACT Inhibition Blocks Induction But Not Maintenance of Pluripotency.Stem cells and development · 2018Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
The mechanisms whereby the crucial pluripotency transcription factor Oct4 regulates target gene expression are incompletely understood. Using an assay system based on partially differentiated embryonic stem cells, we show that Oct4 opposes the accumulation of local H3K9me2 and subsequent Dnmt3a-mediated DNA methylation. Upon binding DNA, Oct4 recruits the histone lysine demethylase Jmjd1c. Chromatin immunoprecipitation (ChIP) time course experiments identify a stepwise Oct4 mechanism involving Jmjd1c recruitment and H3K9me2 demethylation, transient FACT (facilitates chromatin transactions) complex recruitment, and nucleosome depletion. Genome-wide and targeted ChIP confirms binding of newly synthesized Oct4, together with Jmjd1c and FACT, to the Pou5f1 enhancer and a small number of other Oct4 targets, including the Nanog promoter. Histone demethylation is required for both FACT recruitment and H3 depletion. Jmjd1c is required to induce endogenous Oct4 expression and fully reprogram fibroblasts to pluripotency, indicating that the assay system identifies functional Oct4 cofactors. These findings indicate that Oct4 sequentially recruits activities that catalyze histone demethylation and depletion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.