ArticleBMC systems biology2015
Reconstruction and analysis of a genome-scale metabolic model of the oleaginous fungus Mortierella alpina.
Article in BMC systems biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 90 citations in OpenAlex.
- Microbial cell factories for gamma-linolenic acid biosynthesis: metabolic engineering, downstream processing, and future perspectives.Bioresources and bioprocessing · 2026Review
- Developmental synergistic information.Theory in biosciences = Theorie in den Biowissenschaften · 2026Article
- Insights into optimization of oleaginous fungi - genome-scale metabolic reconstruction and analysis ofComputational and structural biotechnology journal · 2025Article
- Characterization of potassium-solubilizing fungi, Mortierella spp., isolated from a poplar plantation rhizosphere soil.Archives of microbiology · 2024Article
- Homologous and Heterologous Expression of Delta(12)-Desaturase inMolecules (Basel, Switzerland) · 2022Article
- Genome-scale metabolic network models: from first-generation to next-generation.Applied microbiology and biotechnology · 2022Review
- Advances in improving the biotechnological application of oleaginous fungus Mortierella alpina.Applied microbiology and biotechnology · 2021Review
- Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri.BMC biotechnology · 2021Article
- Time-resolved multi-omics analysis reveals the role of nutrient stress-induced resource reallocation for TAG accumulation in oleaginous fungusBiotechnology for biofuels · 2020Article
- Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production.BMC biotechnology · 2019Article
- Genomic sequencing, genome-scale metabolic network reconstruction, and in silico flux analysis of the grape endophytic fungus Alternaria sp. MG1.Microbial cell factories · 2019Article
- Article
- Arachidonic acid production by the oleaginous fungusJournal of advanced research · 2018Review
- Reconstruction and Analysis of a Genome-Scale Metabolic Model ofFrontiers in microbiology · 2018Article
- Reconstruction of a Genome-scale Metabolic Network of Komagataeibacter nataicola RZS01 for Cellulose Production.Scientific reports · 2017Article
- IMGMD: A platform for the integration and standardisation of In silico Microbial Genome-scale Metabolic Models.Scientific reports · 2017Article
- Lipid Metabolic Versatility inFrontiers in microbiology · 2017Article
- Microbial production of fatty alcohols.World journal of microbiology & biotechnology · 2016Review
- Reconstruction and analysis of the genome-scale metabolic model of schizochytrium limacinum SR21 for docosahexaenoic acid production.BMC genomics · 2015Article
- Directed optimization of a newly identified squalene synthase from Mortierella alpine based on sequence truncation and site-directed mutagenesis.Journal of industrial microbiology & biotechnology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMortierella alpina is an oleaginous fungus used in the industrial scale production of arachidonic acid (ARA). In order to investigate the metabolic characteristics at a systems level and to explore potential strategies for enhanced lipid production, a genome-scale metabolic model of M. alpina was reconstructed.
resultsThis model included 1106 genes, 1854 reactions and 1732 metabolites. On minimal growth medium, 86 genes were identified as essential, whereas 49 essential genes were identified on yeast extract medium. A series of sequential desaturase and elongase catalysed steps are involved in the synthesis of polyunsaturated fatty acids (PUFAs) from acetyl-CoA precursors, with concomitant NADPH consumption, and these steps were investigated in this study. Oxygen is known to affect the degree of unsaturation of PUFAs, and robustness analysis determined that an oxygen uptake rate of 2.0 mmol gDW(-1) h(-1) was optimal for ARA accumulation. The flux of 53 reactions involving NADPH was significantly altered at different ARA levels. Of these, malic enzyme (ME) was confirmed as a key component in ARA production and NADPH generation. When using minimization of metabolic adjustment, a knock-out of ME led to a 38.28% decrease in ARA production.
conclusionsThe simulation results confirmed the model as a useful tool for future research on the metabolism of PUFAs.
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