ArticleAnesthesiology2015
Using next-generation RNA sequencing to examine ischemic changes induced by cold blood cardioplegia on the human left ventricular myocardium transcriptome.
Article in Anesthesiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
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- Cardiopulmonary bypass with deep hypothermic circulatory arrest results in organ-specific transcriptomic responses in pediatric swine.Translational research : the journal of laboratory and clinical medicine · 2025Article
- Pathophysiological insights into HFpEF from studies of human cardiac tissue.Nature reviews. Cardiology · 2025Review
- Surgical Tumor Resection Deregulates Hallmarks of Cancer in Resected Tissue and the Surrounding Microenvironment.Molecular cancer research : MCR · 2024Article
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- Bone marrow- or adipose-mesenchymal stromal cell secretome preserves myocardial transcriptome profile and ameliorates cardiac damage following ex vivo cold storage.Journal of molecular and cellular cardiology · 2022Article
- Assessment of human bioengineered cardiac tissue function in hypoxic and re-oxygenized environments to understand functional recovery in heart failure.Regenerative therapy · 2021Article
- Uncovering Potential lncRNAs and mRNAs in the Progression From Acute Myocardial Infarction to Myocardial Fibrosis to Heart Failure.Frontiers in cardiovascular medicine · 2021Article
- A Guide to Understanding "State-of-the-Art" Basic Research Techniques in Anesthesiology.Anesthesia and analgesia · 2020Review
- Transcriptome analysis defines myocardium gene signatures in children with ToF and ASD and reveals disease-specific molecular reprogramming in response to surgery with cardiopulmonary bypass.Journal of translational medicine · 2020Article
- Sex differences in gene expression in response to ischemia in the human left ventricular myocardium.Human molecular genetics · 2019Article
- Molecular Genetics of Lidocaine-Containing Cardioplegia in the Human Heart During Cardiac Surgery.The Annals of thoracic surgery · 2018Article
- Integration of Metabonomics and Transcriptomics Reveals the Therapeutic Effects and Mechanisms ofFrontiers in pharmacology · 2018Article
- Article
- Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia.Cardiovascular diabetology · 2017Article
- Increased Atrial β-Adrenergic Receptors and GRK-2 Gene Expression Can Play a Fundamental Role in Heart Failure After Repair of Congenital Heart Disease with Cardiopulmonary Bypass.Pediatric cardiology · 2017Article
- The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle.Circulation. Cardiovascular genetics · 2017Article
- Allele-specific expression in the human heart and its application to postoperative atrial fibrillation and myocardial ischemia.Genome medicine · 2016Article
- Loss of RNA expression and allele-specific expression associated with congenital heart disease.Nature communications · 2016Article
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThe exact mechanisms that underlie the pathological processes of myocardial ischemia in humans are unclear. Cardiopulmonary bypass with cardioplegic arrest allows the authors to examine the whole transcriptional profile of human left ventricular myocardium at baseline and after exposure to cold cardioplegia-induced ischemia as a human ischemia model.
methodsThe authors obtained biopsies from 45 patients undergoing aortic valve replacement surgery at baseline and after an average of 79 min of cold cardioplegic arrest. Samples were RNA sequenced and analyzed with the Partek Genomics Suite (Partek Inc., St. Louis, MO) for differential expression. Ingenuity Pathway Analysis (Ingenuity Systems, Redwood City, CA) and Biobase ExPlain (Biobase GmbH, Wolfenbuettel, Germany) systems were used for functional and pathway analyses.
resultsOf the 4,098 genes with a mean expression value greater than 5, 90% were down-regulated and 9.1% were up-regulated. Of those, 1,241 were significantly differentially expressed. Gene ontology analysis revealed significant down-regulation in immune inflammatory response and complement activation categories and highly consistent was the down-regulation of intelectin 1, proteoglycan, and secretory leukocyte peptidase inhibitor. Up-regulated genes of interest were FBJ murine osteosarcoma viral oncogene homolog and the hemoglobin genes hemoglobin α1 (HBA1) and hemoglobin β. In addition, analysis of transcription factor-binding sites revealed interesting targets in factors regulating reactive oxygen species production, apoptosis, immunity, cytokine production, and inflammatory response.
conclusionsThe authors have shown that the human left ventricle exhibits significant changes in gene expression in response to cold cardioplegia-induced ischemia during cardiopulmonary bypass, which provides great insight into the pathophysiology of ventricular ischemia, and thus, may help guide efforts to reduce myocardial damage during surgery.
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