ArticleJournal of biomechanical engineering2015
Epigenetic changes during mechanically induced osteogenic lineage commitment.
Article in Journal of biomechanical engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Cellular stress and epigenetic regulation in adult stem cells.Life science alliance · 2024Review
- Research progress on the regulatory mechanism of integrin-mediated mechanical stress in cells involved in bone metabolism.Journal of cellular and molecular medicine · 2024Review
- Fenoldopam Sensitizes Primary Cilia-Mediated Mechanosensing to Promote Osteogenic Intercellular Signaling and Whole Bone Adaptation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- Human osteoblasts obtained from distinct periarticular sites demonstrate differences in biological function in vitro.Bone & joint research · 2021Article
- Chromium Oxide Nanoparticle Impaired Osteogenesis and Cellular Response to Mechanical Stimulus.International journal of nanomedicine · 2021Article
- Histone demethylase KDM4A regulates adipogenic and osteogenic differentiation via epigenetic regulation of C/EBPα and canonical Wnt signaling.Cellular and molecular life sciences : CMLS · 2020Article
- DNA methylation and demethylation link the properties of mesenchymal stem cells: Regeneration and immunomodulation.World journal of stem cells · 2020Review
- Article
- Feasibility, potency, and safety of growing human mesenchymal stem cells in space for clinical application.NPJ microgravity · 2020Article
- Primary cilia: Cell and molecular mechanosensors directing whole tissue function.Seminars in cell & developmental biology · 2017Review
- Epigenome alterations in aortic valve stenosis and its related left ventricular hypertrophy.Clinical epigenetics · 2017Review
- [Role of DNA methylation in regulation of osteogenic differentiation of stem cells].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2016Article
- Biocompatibility of Polypyrrole with Human Primary Osteoblasts and the Effect of Dopants.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Osteogenic lineage commitment is often evaluated by analyzing gene expression. However, many genes are transiently expressed during differentiation. The availability of genes for expression is influenced by epigenetic state, which affects the heterochromatin structure. DNA methylation, a form of epigenetic regulation, is stable and heritable. Therefore, analyzing methylation status may be less temporally dependent and more informative for evaluating lineage commitment. Here we analyzed the effect of mechanical stimulation on osteogenic differentiation by applying fluid shear stress for 24 hr to osteocytes and then applying the osteocyte-conditioned medium (CM) to progenitor cells. We analyzed gene expression and changes in DNA methylation after 24 hr of exposure to the CM using quantitative real-time polymerase chain reaction and bisulfite sequencing. With fluid shear stress stimulation, methylation decreased for both adipogenic and osteogenic markers, which typically increases availability of genes for expression. After only 24 hr of exposure to CM, we also observed increases in expression of later osteogenic markers that are typically observed to increase after seven days or more with biochemical induction. However, we observed a decrease or no change in early osteogenic markers and decreases in adipogenic gene expression. Treatment of a demethylating agent produced an increase in all genes. The results indicate that fluid shear stress stimulation rapidly promotes the availability of genes for expression, but also specifically increases gene expression of later osteogenic markers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.