ArticleJournal of biomedical optics2015
Optical coherence tomography guided microinjections in live mouse embryos: high-resolution targeted manipulation for mouse embryonic research.
Article in Journal of biomedical optics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Mouse embryo phenotyping with optical coherence tomography.Frontiers in cell and developmental biology · 2022Review
- Embryonic Mouse Cardiodynamic OCT Imaging.Journal of cardiovascular development and disease · 2020Review
- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- Speckle variance optical coherence tomography of blood flow in the beating mouse embryonic heart.Journal of biophotonics · 2017Article
- Dynamic imaging and quantitative analysis of cranial neural tube closure in the mouse embryo using optical coherence tomography.Biomedical optics express · 2017Article
- Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography.Journal of biophotonics · 2016Article
- SMAD Signaling Is Required for Structural Integrity of the Female Reproductive Tract and Uterine Function During Early Pregnancy in Mice.Biology of reproduction · 2016Article
- Applicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography.Biomedical optics express · 2016Article
- Optical coherence tomography for embryonic imaging: a review.Journal of biomedical optics · 2016Review
- High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography.Biomedical optics express · 2015Article
- Algorithms for improved 3-D reconstruction of live mammalian embryo vasculature from optical coherence tomography data.Quantitative imaging in medicine and surgery · 2015Article
- Live four-dimensional optical coherence tomography reveals embryonic cardiac phenotype in mouse mutant.Journal of biomedical optics · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The ability to conduct highly localized delivery of contrast agents, viral vectors, therapeutic or pharmacological agents, and signaling molecules or dyes to live mammalian embryos is greatly desired to enable a variety of studies in the field of developmental biology, such as investigating the molecular regulation of cardiovascular morphogenesis. To meet such a demand, we introduce, for the first time, the concept of employing optical coherence tomography (OCT)-guide microinjections in live mouse embryos, which provides precisely targeted manipulation with spatial resolution at the micrometer scale. The feasibility demonstration is performed with experimental studies on cultured live mouse embryos at E8.5 and E9.5. Additionally, we investigate the OCT-guided microinjection of gold–silica nanoshells to the yolk sac vasculature of live cultured mouse embryos at the stage when the heart just starts to beat, as a potential approach for dynamic assessment of cardiovascular form and function before the onset of blood cell circulation. Also, the capability of OCT to quantitatively monitor and measure injection volume is presented. Our results indicate that OCT-guided microinjection could be a useful tool for mouse embryonic research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.