ArticleACS applied materials & interfaces2015
Detection of viruses by counting single fluorescent genetically biotinylated reporter immunophage using a lateral flow assay.
Article in ACS applied materials & interfaces, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04450667 (Impact of Preoperative Oral Rehydration on the Incidence of Spinal Anesthesia-induced Hypotension for Scheduled Cesarean Section.), which is not on this map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Impact of Preoperative Oral Rehydration on the Incidence of Spinal Anesthesia-induced Hypotension for Scheduled Cesarean Section.
Who cites it
8 citing papers in PubMed, 25 citations in OpenAlex.
- Engineered bacteriophage-based bioimaging Technology: Development and applications.Synthetic and systems biotechnology · 2025Review
- Smartphone-read phage lateral flow assay for point-of-care detection of infection.The Analyst · 2023Article
- Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Expanding the chemical diversity of M13 bacteriophage.Frontiers in microbiology · 2022Review
- Increasing Binding Efficiency via Reporter Shape and Flux in a Viral Nanoparticle Lateral-Flow Assay.ACS applied materials & interfaces · 2017Article
- Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay.The Analyst · 2016Article
- Chemically Modifying Viruses for Diverse Applications.ACS chemical biology · 2016Review
- Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays.Analytical chemistry · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
We demonstrated a lateral flow immunoassay (LFA) for detection of viruses using fluorescently labeled M13 bacteriophage as reporters and single-reporter counting as the readout. AviTag-biotinylated M13 phage were functionalized with antibodies using avidin-biotin conjugation and fluorescently labeled with AlexaFluor 555. Individual phage bound to target viruses (here MS2 as a model) captured on an LFA membrane strip were imaged using epi-fluorescence microscopy. Using automated image processing, we counted the number of bound phage in micrographs as a function of target concentration. The resultant assay was more sensitive than enzyme-linked immunosorbent assays and traditional colloidal-gold nanoparticle LFAs for direct detection of viruses.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.