ArticlePloS one2015
Optical clearing in dense connective tissues to visualize cellular connectivity in situ.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Loss of type III collagen has limited effect on tendon healing in young adult mice.Matrix biology : journal of the International Society for Matrix Biology · 2026Article
- 3D immunolabelling of human aortic valves by optimized CUBIC tissue clearing and its application for neural mapping in aortic stenosis.European heart journal open · 2026Article
- Rapid sonication-assisted whole tissue clearing and immunostaining.Scientific reports · 2025Article
- Extracellular matrix deposition precedes muscle-tendon integration during murine forelimb morphogenesis.Communications biology · 2025Article
- Mechanical loading is required for initiation of extracellular matrix deposition at the developing murine myotendinous junction.Matrix biology : journal of the International Society for Matrix Biology · 2023Article
- Review
- Application of 3D MAPs pipeline identifies the morphological sequence chondrocytes undergo and the regulatory role of GDF5 in this process.Nature communications · 2021Article
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- Review
- Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography.Biological procedures online · 2020Article
- A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning.Bio-protocol · 2019Article
- Computational Modeling of Developing Cartilage Using Experimentally Derived Geometries and Compressive Moduli.Journal of biomechanical engineering · 2019Article
- FxClear, A Free-hydrogel Electrophoretic Tissue Clearing Method for Rapid De-lipidation of Tissues with High Preservation of Immunoreactivity.Experimental neurobiology · 2019Article
- Tissue Clearing and Its Application to Bone and Dental Tissues.Journal of dental research · 2019Review
- Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning.eLife · 2019Article
- New artery of knowledge: 3D models of angiogenesis.Vascular biology (Bristol, England) · 2019Review
- Immunolabeling of Cleared Human Pancreata Provides Insights into Three-Dimensional Pancreatic Anatomy and Pathology.The American journal of pathology · 2018Article
- Three-dimensional visualization of extracellular matrix networks during murine development.Developmental biology · 2018Article
- Functional MRI can detect changes in intratissue strains in a full thickness and critical sized ovine cartilage defect model.Journal of biomechanics · 2018Article
- Image-based modelling of skeletal muscle oxygenation.Journal of the Royal Society, Interface · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Visualizing the three-dimensional morphology and spatial patterning of cells embedded deep within dense connective tissues of the musculoskeletal system has been possible only by utilizing destructive techniques. Here we utilize fructose-based clearing solutions to image cell connectivity and deep tissue-scale patterning in situ by standard confocal microscopy. Optical clearing takes advantage of refractive index matching of tissue and the embedding medium to visualize light transmission through a broad range of bovine and whole mount murine tissues, including cartilage, bone, and ligament, of the head and hindlimb. Using non-destructive methods, we show for the first time intercellular chondrocyte connections throughout the bulk of cartilage, and we reveal in situ patterns of osteocyte processes and the lacunar-canalicular system deep within mineralized cortical bone. Optical clearing of connective tissues is expected to find broad application for the study of cell responses in normal physiology and disease pathology.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.