Effect of the polydispersity of RBCs on the recovery rate of RBCs during the removal of CPAs.

Heyuan Qiao, Weiping Ding, Yuncong Ma, Sijie Sun, Dayong Gao

Abstract read

In one paragraph

Article in Computational and mathematical methods in medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Heyuan QiaoCenter for Biomedical Engineering, University of Science and Technology of China, Hefei, Anhui 230027, China ; Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.ORCID 0000-0002-8351-1425

Weiping DingCenter for Biomedical Engineering, University of Science and Technology of China, Hefei, Anhui 230027, China ; Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.ORCID 0000-0002-3331-1011

Yuncong MaCenter for Biomedical Engineering, University of Science and Technology of China, Hefei, Anhui 230027, China ; Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.

Sijie SunDepartment of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.

Dayong GaoDepartment of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the process of removing cryoprotectants from cryopreserved blood, the theoretically optimal operating condition, which is based on the assumption that the distribution of red blood cells is uniform, is often used to reduce or even avoid the hypotonic damage to cells. However, due to the polydispersity of cells, the optimal condition is actually not reliable. In this study, based on the discrete concept developed in our previous work, the effect of the polydispersity on the recovery rate of cells in the dilution-filtration system was statistically investigated by assigning three random parameters, isotonic cell volume, cell surface area, and osmotically inactive cell volume, to cells in small units of blood. The results show that, due to the polydispersity, the real recovery rate deviates from the ideal value that is based on uniform distribution. The deviation significantly increases with the standard errors of cell parameters, and it can be also magnified by high cryoprotectant concentrations. Under the effect of polydispersity, the uniform distribution-based optimized blood or diluent flow rate is not perfect. In practice, one should adopt a more conservative blood or diluent flow rate so that the hypotonic damage to cells can be further reduced.

Indexed as

AlgorithmsBlood Flow VelocityBlood PreservationCell SizeCell SurvivalCryopreservationCryoprotective AgentsErythrocytesFiltrationHumansOsmosisReproducibility of ResultsSpecimen HandlingCryoprotective Agents

Identifiers

PMID25580156

DOI10.1155/2014/792302

PMCPMC4279270

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.