Evidence map›Paper›PMID 25579786›Full record

ArticleAnalytical biochemistry2015

Determination of hyaluronan molecular mass distribution in human breast milk.

Han Yuan, Ripal Amin, Xin Ye, Carol A de la Motte, Mary K Cowman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 40 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Methods for isolating and analyzing physiological hyaluronan: a review.American journal of physiology. Cell physiology · 2022
    Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Multifunctional Role of 35 Kilodalton Hyaluronan in Promoting Defense of the Intestinal Epithelium.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018
    Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Han YuanDepartment of Chemical and Biomolecular Engineering, Polytechnic School of Engineering, New York University, Brooklyn, NY 11201, USA.
Ripal AminDepartment of Chemical and Biomolecular Engineering, Polytechnic School of Engineering, New York University, Brooklyn, NY 11201, USA; Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA; Department of Colorectal Surgery, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Xin YeDepartment of Chemical and Biomolecular Engineering, Polytechnic School of Engineering, New York University, Brooklyn, NY 11201, USA.
Carol A de la MotteDepartment of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA; Department of Colorectal Surgery, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Mary K CowmanDepartment of Chemical and Biomolecular Engineering, Polytechnic School of Engineering, New York University, Brooklyn, NY 11201, USA. Electronic address: mary.cowman@nyu.edu.
New York University · US

Funding

VERSICAN PROTEOLYSIS AND REGULATION OF VASCULAR SMOOTH MUSCLEP01HL107147 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI LAUER, MARK E · 2011 to 2017
$16.1M
Hyaluronan regulation of microbial host defense of the intestineR01HD061918 · NICHD · CLEVELAND CLINIC LERNER COM-CWRU · PI DE LA MOTTE, CAROL A. · 2009 to 2013
$2.0M
NHLBI NIH HHS P01 HL107147NICHD NIH HHS R01 HD061918
6 · The paper itself

Abstract

Hyaluronan (HA) in human milk mediates host responses to microbial infection via TLR4- and CD44-dependent signaling. Signaling by HA is generally size specific. Because pure HA with average molecular mass (M) of 35 kDa can elicit a protective response in intestinal epithelial cells, it has been proposed that human milk HA may have a bioactive low-M component. Here we report the size distribution of HA in human milk samples from 20 unique donors. A new method for HA analysis, employing ion exchange (IEX) chromatography to fractionate HA by size and specific quantification of each size fraction by competitive enzyme-linked sorbent assay (ELSA), was developed. When separated into four fractions, milk HA with M⩽20 kDa, M∼20 to 60 kDa, and M∼60 to 110 kDa comprised averages of 1.5, 1.4, and 2.0% of the total HA, respectively. The remaining 95% was HA with M⩾110 kDa. Electrophoretic analysis of the higher M HA from 13 samples showed nearly identical M distributions, with an average M of approximately 440 kDa. This higher M HA component in human milk is proposed to bind to CD44 and to enhance human beta defensin 2 (HBD2) induction by the low-M HA components.

Indexed as

CalibrationChemical FractionationDensitometryElectrophoresisEnzyme-Linked Immunosorbent AssayFemaleHumansHyaluronic AcidIon ExchangeMilk, HumanMolecular WeightHyaluronic AcidElectrophoresisHyaluronanIon exchangeMilkMolecular massQuantification

Identifiers

PMID25579786
PMCPMC4357551
OpenAlexW1998487662

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.