ArticleApplied and environmental microbiology2015
Efficient metabolic exchange and electron transfer within a syntrophic trichloroethene-degrading coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Mechanisms of microbial co-aggregation in mixed anaerobic cultures.Applied microbiology and biotechnology · 2024Review
- Metabolite cross-feeding enables concomitant catabolism of chlorinated methanes and chlorinated ethenes in synthetic microbial assemblies.The ISME journal · 2024Article
- Syntrophic Interactions Ameliorate Arsenic Inhibition of Solvent-DechlorinatingEnvironmental science & technology · 2023Article
- Recent advances and trends of trichloroethylene biodegradation: A critical review.Frontiers in microbiology · 2022Review
- Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene.The ISME journal · 2021Article
- Acetylene-Fueled Trichloroethene Reductive Dechlorination in a Groundwater Enrichment Culture.mBio · 2021Article
- Iron Sulfide Enhanced the Dechlorination of Trichloroethene byFrontiers in microbiology · 2021Article
- Effects of Arsenic on Trichloroethene-Dechlorination Activities ofEnvironmental science & technology · 2020Article
- Synthetic microbial consortia for biosynthesis and biodegradation: promises and challenges.Journal of industrial microbiology & biotechnology · 2019Review
- Structural dynamics and transcriptomic analysis of Dehalococcoides mccartyi within a TCE-Dechlorinating community in a completely mixed flow reactor.Water research · 2019Article
- An integrated meta-omics approach reveals substrates involved in synergistic interactions in a bisphenol A (BPA)-degrading microbial community.Microbiome · 2019Article
- Hydrogen production by Sulfurospirillum species enables syntrophic interactions of Epsilonproteobacteria.Nature communications · 2018Article
- Acetylenotrophy: a hidden but ubiquitous microbial metabolism?FEMS microbiology ecology · 2018Review
- Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene byFrontiers in microbiology · 2018Article
- Detection of Diazotrophy in the Acetylene-Fermenting Anaerobe Pelobacter sp. Strain SFB93.Applied and environmental microbiology · 2017Article
- Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities.Applied and environmental microbiology · 2017Article
- Acetylene Fuels TCE Reductive Dechlorination by Defined Dehalococcoides/Pelobacter Consortia.Environmental science & technology · 2017Article
- Article
- Membrane Complexes ofFrontiers in microbiology · 2016Article
- Indirect Evidence Link PCB Dehalogenation with Geobacteraceae in Anaerobic Sediment-Free Microcosms.Frontiers in microbiology · 2016Article
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Abstract
Dehalococcoides mccartyi 195 (strain 195) and Syntrophomonas wolfei were grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. The maximum dechlorination rate (9.9 ± 0.1 μmol day(-1)) and cell yield [(1.1 ± 0.3) × 10(8) cells μmol(-1) Cl(-)] of strain 195 maintained in coculture were, respectively, 2.6 and 1.6 times higher than those measured in the pure culture. The strain 195 cell concentration was about 16 times higher than that of S. wolfei in the coculture. Aqueous H2 concentrations ranged from 24 to 180 nM during dechlorination and increased to 350 ± 20 nM when TCE was depleted, resulting in cessation of butyrate fermentation by S. wolfei with a theoretical Gibbs free energy of -13.7 ± 0.2 kJ mol(-1). Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. The minimum H2 threshold value for TCE dechlorination by strain 195 in the coculture was 0.6 ± 0.1 nM. Cell aggregates during syntrophic growth were observed by scanning electron microscopy. The interspecies distances to achieve H2 fluxes required to support the measured dechlorination rates were predicted using Fick's law and demonstrated the need for aggregation. Filamentous appendages and extracellular polymeric substance (EPS)-like structures were present in the intercellular spaces. The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels.
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