ArticleEukaryotic cell2015
Dynamics of the lipid droplet proteome of the Oleaginous yeast rhodosporidium toruloides.
Article in Eukaryotic cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- EngineeringSynthetic and systems biotechnology · 2026Article
- Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2026Article
- Rhodosporidium toruloides-a new surrogate model to study rapamycin induced effects on human aging and cancer.Cellular and molecular life sciences : CMLS · 2025Article
- Proteomic insights into the physiology and metabolism of oleaginous yeasts and filamentous fungi.Frontiers in microbiology · 2025Review
- A fluorescence lifetime-based novel method for accurate lipid quantification of BODIPY vital-stained C. elegans.Journal of lipid research · 2024Article
- Caleosin/peroxygenases: multifunctional proteins in plants.Annals of botany · 2023Review
- Adaptation of Proteome and Metabolism in Different Haplotypes ofMicroorganisms · 2023Article
- A Perilipin Affects Lipid Droplet Homeostasis and Aerial Hyphal Growth, but Has Only Small Effects on Virulence in the Insect Pathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Genetic manipulation of the interconversion between diacylglycerols and triacylglycerols inFrontiers in bioengineering and biotechnology · 2022Article
- Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast.Bioresources and bioprocessing · 2021Review
- Integrating transcriptomic and metabolomic analysis of the oleaginous yeast Rhodosporidium toruloides IFO0880 during growth under different carbon sources.Applied microbiology and biotechnology · 2021Article
- Potassium and Sodium Salt Stress Characterization in the Yeasts Saccharomyces cerevisiae, Kluyveromyces marxianus, andApplied and environmental microbiology · 2021Article
- Engineering oleaginous yeast Rhodotorula toruloides for overproduction of fatty acid ethyl esters.Biotechnology for biofuels · 2021Article
- Engineering the Oleaginous YeastFrontiers in bioengineering and biotechnology · 2021Article
- Rhodosporidium toruloides - A potential red yeast chassis for lipids and beyond.FEMS yeast research · 2020Review
- The Puzzling Conservation and Diversification of Lipid Droplets from Bacteria to Eukaryotes.Results and problems in cell differentiation · 2020Article
- Proteomics Analysis of Lipid Droplets from the Oleaginous Alga Chromochloris zofingiensis Reveals Novel Proteins for Lipid Metabolism.Genomics, proteomics & bioinformatics · 2019Article
- Monoterpene production by the carotenogenic yeast Rhodosporidium toruloides.Microbial cell factories · 2019Article
- Simultaneous lipid biosynthesis and recovery for oleaginous yeastBiotechnology for biofuels · 2019Article
- Proteome analysis of xylose metabolism inBiotechnology for biofuels · 2019Article
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid droplets (LDs) are ubiquitous organelles that serve as a neutral lipid reservoir and a hub for lipid metabolism. Manipulating LD formation, evolution, and mobilization in oleaginous species may lead to the production of fatty acid-derived biofuels and chemicals. However, key factors regulating LD dynamics remain poorly characterized. Here we purified the LDs and identified LD-associated proteins from cells of the lipid-producing yeast Rhodosporidium toruloides cultured under nutrient-rich, nitrogen-limited, and phosphorus-limited conditions. The LD proteome consisted of 226 proteins, many of which are involved in lipid metabolism and LD formation and evolution. Further analysis of our previous comparative transcriptome and proteome data sets indicated that the transcription level of 85 genes and protein abundance of 77 proteins changed under nutrient-limited conditions. Such changes were highly relevant to lipid accumulation and partially confirmed by reverse transcription-quantitative PCR. We demonstrated that the major LD structure protein Ldp1 is an LD marker protein being upregulated in lipid-rich cells. When overexpressed in Saccharomyces cerevisiae, Ldp1 localized on the LD surface and facilitated giant LD formation, suggesting that Ldp1 plays an important role in controlling LD dynamics. Our results significantly advance the understanding of the molecular basis of lipid overproduction and storage in oleaginous yeasts and will be valuable for the development of superior lipid producers.
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