ArticleAnaerobe2015
An alkaline phosphatase reporter for use in Clostridium difficile.
Article in Anaerobe, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- CenIR, an essential BlaIR-family regulatory system inJournal of bacteriology · 2026Article
- CenIR, an essential BlaIR-family regulatory system inbioRxiv : the preprint server for biology · 2026Article
- Nanoluciferase-Based Reporters for Clostridioides difficile.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Characterization of two co-regulated response regulators inJournal of bacteriology · 2025Article
- Article
- A metabolite dehydrogenase pathway represses sporulation of Clostridioides difficile.Anaerobe · 2025Article
- Phase Variation of Flagella and Toxins in Clostridioides difficile is Mediated by Selective Rho-dependent Termination.Journal of molecular biology · 2024Article
- The pH-responsive SmrR-SmrT system modulatesInfection and immunity · 2024Article
- Glycine fermentation byInfection and immunity · 2023Article
- The RgaS-RgaR two-component system promotes Clostridioides difficile sporulation through a small RNA and the Agr1 system.PLoS genetics · 2023Article
- The RgaS-RgaR two-component system promotesbioRxiv : the preprint server for biology · 2023Article
- Coordinated modulation of multiple processes through phase variation of a c-di-GMP phosphodiesterase in Clostridioides difficile.PLoS pathogens · 2022Article
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- Article
- Type I toxin-antitoxin systems contribute to the maintenance of mobile genetic elements in Clostridioides difficile.Communications biology · 2020Article
- Article
- Rho factor mediates flagellum and toxin phase variation and impacts virulence in Clostridioides difficile.PLoS pathogens · 2020Article
- Strain-Dependent RstA Regulation of Clostridioides difficile Toxin Production and Sporulation.Journal of bacteriology · 2020Article
- Regulation and Anaerobic Function of theAntimicrobial agents and chemotherapy · 2019Article
- Clostridial Genetics: Genetic Manipulation of the Pathogenic Clostridia.Microbiology spectrum · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Clostridium difficile is an anaerobic, Gram-positive pathogen that causes severe gastrointestinal disease in humans and other mammals. C. difficile is notoriously difficult to work with and, until recently, few tools were available for genetic manipulation and molecular analyses. Despite the recent advances in the field, there is no simple or cost-effective technique for measuring gene transcription in C. difficile other than direct transcriptional analyses (e.g., quantitative real-time PCR and RNA-seq), which are time-consuming, expensive and difficult to scale-up. We describe the development of an in vivo reporter assay that can provide qualitative and quantitative measurements of C. difficile gene expression. Using the Enterococcus faecalis alkaline phosphatase gene, phoZ, we measured expression of C. difficile genes using a colorimetric alkaline phosphatase assay. We show that inducible alkaline phosphatase activity correlates directly with native gene expression. The ability to analyze gene expression using a standard reporter is an important and critically needed tool to study gene regulation and design genetic screens for C. difficile and other anaerobic clostridia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.