ArticleApplied microbiology and biotechnology2015
Isolation and characterization of novel lipases/esterases from a bovine rumen metagenome.
Article in Applied microbiology and biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Metagenomic Identification and Characterization of Novel Vitamin BMicroorganisms · 2025Article
- Identification of Cutinolytic Esterase from Microplastic-Associated Microbiota Using Functional Metagenomics and Its Plastic Degrading Potential.Molecular biotechnology · 2024Article
- Microbiome-derived antimicrobial peptides show therapeutic activity against the critically important priority pathogen, Acinetobacter baumannii.NPJ biofilms and microbiomes · 2024Article
- Effects of Coconut Oil and Palm Oil on Growth, Rumen Microbiota, and Fatty Acid Profile of Suckling Calves.Microorganisms · 2023Article
- Anti-Frontiers in microbiology · 2023Review
- Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.Microbiome · 2022Article
- In silico identification of two peptides with antibacterial activity against multidrug-resistant Staphylococcus aureus.NPJ biofilms and microbiomes · 2022Article
- Discovery of novel carbohydrate degrading enzymes from soda lakes through functional metagenomics.Frontiers in microbiology · 2022Article
- Gossypol detoxification in the rumen andAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- An uncharacterized protein from the metagenome with no obvious homology to known lipases shows excellent alkaline lipase properties and potential applications in the detergent industry.Biotechnology letters · 2021Article
- Rumen Fermentation, Digestive Enzyme Activity, and Bacteria Composition between Pre-Weaning and Post-Weaning Dairy Calves.Animals : an open access journal from MDPI · 2021Article
- Article
- Dietary Supplementation of Lysophospholipids Affects Feed Digestion in Lambs.Animals : an open access journal from MDPI · 2019Article
- Characterization of a Novel Moderately Thermophilic Solvent-Tolerant Esterase Isolated From a Compost Metagenome Library.Frontiers in microbiology · 2019Article
- Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference.Microbial cell factories · 2018Article
- Enzymatic Study of Linoleic and Alpha-Linolenic Acids Biohydrogenation by Chloramphenicol-Treated Mixed Rumen Bacterial Species.Frontiers in microbiology · 2018Article
- Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families.Scientific reports · 2017Article
- Functional Metagenomics as a Tool for Identification of New Antibiotic Resistance Genes from Natural Environments.Microbial ecology · 2017Review
- Bioprospecting hot spring metagenome: lipase for the production of biodiesel.Environmental science and pollution research international · 2017Article
- Application of meta-omics techniques to understand greenhouse gas emissions originating from ruminal metabolism.Genetics, selection, evolution : GSE · 2017Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improving the health beneficial fatty acid content of meat and milk is a major challenge requiring an increased understanding of rumen lipid metabolism. In this study, we isolated and characterized rumen bacterial lipases/esterases using functional metagenomics. Metagenomic libraries were constructed from DNA extracted from strained rumen fluid (SRF), solid-attached bacteria (SAB) and liquid-associated rumen bacteria (LAB), ligated into a fosmid vector and subsequently transformed into an Escherichia coli host. Fosmid libraries consisted of 7,744; 8,448; and 7,680 clones with an average insert size of 30 to 35 kbp for SRF, SAB and LAB, respectively. Transformants were screened on spirit blue agar plates containing tributyrin for lipase/esterase activity. Five SAB and four LAB clones exhibited lipolytic activity, and no positive clones were found in the SRF library. Fosmids from positive clones were pyrosequenced and twelve putative lipase/esterase genes and two phospholipase genes retrieved. Although the derived proteins clustered into diverse esterase and lipase families, a degree of novelty was seen, with homology ranging from 40 to 78% following BlastP searches. Isolated lipases/esterases exhibited activity against mostly short- to medium-chain substrates across a range of temperatures and pH. The function of these novel enzymes recovered in ruminal metabolism needs further investigation, alongside their potential industrial uses.
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