ArticleThe Journal of biological chemistry2015
Biochemical characterization and structural analysis of a bifunctional cellulase/xylanase from Clostridium thermocellum.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- A Thermostable Multifunctional GH5 Endoglucanase From Bacillus sp. for Lignocellulosic Biomass Saccharification.Biotechnology journal · 2026Article
- Discovery and engineering of bifunctional enzymes for lignocellulose degradation: Metagenomic and computational approaches.Biotechnology reports (Amsterdam, Netherlands) · 2025Review
- Non-Catalytic Domains of Glycoside Hydrolase Family 5 fromJournal of microbiology and biotechnology · 2025Article
- A trimeric glycosylated GH45 cellulase from the red abalone (Haliotis rufescens) exhibits endo and exoactivity.PloS one · 2024Article
- Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets.ACS catalysis · 2023Article
- Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Insights into promiscuous chitosanases: the known and the unknown.Applied microbiology and biotechnology · 2022Review
- Characterization of a novel thermophilic metagenomic GH5 endoglucanase heterologously expressed in Escherichia coli and Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2022Article
- Biochemical characterization and cleavage pattern analysis of a novel chitosanase with cellulase activity.Applied microbiology and biotechnology · 2022Article
- Multifunctional cellulases are potent, versatile tools for a renewable bioeconomy.Current opinion in biotechnology · 2021Review
- A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.The Journal of biological chemistry · 2020Article
- Enzymatic degradation of plant biomass and synthetic polymers.Nature reviews. Chemistry · 2020Review
- Extent and Origins of Functional Diversity in a Subfamily of Glycoside Hydrolases.Journal of molecular biology · 2019Article
- A novel thermostable GH10 xylanase with activities on a wide variety of cellulosic substrates from a xylanolyticBiotechnology for biofuels · 2019Article
- Biochemical Characterization of a Novel Monospecific Endo-β-1,4-Glucanase Belonging to GH Family 5 From a Rhizosphere Metagenomic Library.Frontiers in microbiology · 2019Article
- The GH10 and GH48 dual-functional catalytic domains from a multimodular glycoside hydrolase synergize in hydrolyzing both cellulose and xylan.Biotechnology for biofuels · 2019Article
- Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein.BMC biotechnology · 2018Article
- A bifunctional cellulase-xylanase of a new Chryseobacterium strain isolated from the dung of a straw-fed cattle.Microbial biotechnology · 2018Article
- Enhancing the catalytic activity of a novel GH5 cellulaseBiotechnology for biofuels · 2018Article
- Bifunctional recombinant cellulase-xylanase (rBhcell-xyl) from the polyextremophilic bacterium Bacillus halodurans TSLV1 and its utility in valorization of renewable agro-residues.Extremophiles : life under extreme conditions · 2016Article
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We expressed an active form of CtCel5E (a bifunctional cellulase/xylanase from Clostridium thermocellum), performed biochemical characterization, and determined its apo- and ligand-bound crystal structures. From the structures, Asn-93, His-168, His-169, Asn-208, Trp-347, and Asn-349 were shown to provide hydrogen-bonding/hydrophobic interactions with both ligands. Compared with the structures of TmCel5A, a bifunctional cellulase/mannanase homolog from Thermotoga maritima, a flexible loop region in CtCel5E is the key for discriminating substrates. Moreover, site-directed mutagenesis data confirmed that His-168 is essential for xylanase activity, and His-169 is more important for xylanase activity, whereas Asn-93, Asn-208, Tyr-270, Trp-347, and Asn-349 are critical for both activities. In contrast, F267A improves enzyme activities.
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