ArticleBiomedical optics express2014
Fast monitoring of in-vivo conformational changes in myosin using single scan polarization-SHG microscopy.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Characterization of inner ear granular bodies by polarization-resolved second harmonic generation microscopy.Biomedical optics express · 2026Article
- Double Stokes polarimetric microscopy for chiral fibrillar aggregates.Scientific reports · 2025Article
- Polarimetric second harmonic generation microscopy of partially oriented fibers II: Imaging study.Biophysical journal · 2023Article
- Polarimetric second-harmonic generation microscopy of partially oriented fibers I: Digital modeling.Biophysical journal · 2023Article
- Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generation.Life science alliance · 2023Article
- PSHG-TISS: A collection of polarization-resolved second harmonic generation microscopy images of fixed tissues.Scientific data · 2022Article
- Influence of hematoxylin and eosin staining on the quantitative analysis of second harmonic generation imaging of fixed tissue sections.Biomedical optics express · 2021Article
- Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy.Biophysical journal · 2020Article
- Changes in the crystallographic structures of cardiac myosin filaments detected by polarization-dependent second harmonic generation microscopy.Biomedical optics express · 2019Article
- Comparison of Different Polarization Sensitive Second Harmonic Generation Imaging Techniques.Methods and protocols · 2019Article
- Polarization-resolved second harmonic microscopy of skeletal muscle in sepsis.Biomedical optics express · 2018Article
- μMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure.Scientific reports · 2017Article
- Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry.Scientific reports · 2017Article
- Molecular Order of Arterial Collagen Using Circular Polarization Second-Harmonic Generation Imaging.Biophysical journal · 2016Article
- Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution.Biomedical optics express · 2016Article
- Translational label-free nonlinear imaging biomarkers to classify the human corneal microstructure.Biomedical optics express · 2015Article
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3 authors.
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Abstract
Fast imaging of molecular changes under high-resolution and label-free conditions are essential for understanding in-vivo processes, however, current techniques are not able to monitor such changes in real time. Polarization sensitive second harmonic generation (PSHG) imaging is a minimally invasive optical microscopy technique capable of quantifying molecular conformational changes occurring below the diffraction limit. Up to now, such information is generally retrieved by exciting the sample with different linear polarizations. This procedure requires the sample to remain static during measurements (from a few second to minutes), preventing the use of PSHG microscopy from studying moving samples or molecular dynamics in living organisms. Here we demonstrate an imaging method that is one order of magnitude faster than conventional PSHG. Based on circular polarization excitation and instantaneous polarimetry analysis of the second harmonic signal generated in the tissue, the method is able to instantaneously obtain molecular information within a pixel dwell time. As a consequence, a single scan is only required to retrieve all the information. This allowed us to perform PSHG imaging in moving C. elegans, monitoring myosin's dynamics during the muscular contraction and relaxation. Since the method provides images of the molecular state, an unprecedented global understanding of the muscles dynamics is possible by correlating changes in different regions of the sample.
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