Evidence map›Paper›PMID 25574430›Full record

ArticleBiomedical optics express2014

Simultaneous decomposition of multiple hyperspectral data sets: signal recovery of unknown fluorophores in the retinal pigment epithelium.

R Theodore Smith, Robert Post, Ansh Johri, Michele D Lee, Zsolt Ablonczy, Christine A Curcio, Thomas Ach, Paul Sajda

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Hyperspectral Imaging and the Retina: Worth the Wave?Translational vision science & technology · 2020
    Pooled it
  2. Article
  3. Review
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  12. Review
  13. Bestrophinopathy: An RPE-photoreceptor interface disease.Progress in retinal and eye research · 2017
    Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Translational Retinal Imaging.Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

R Theodore SmithDepartment of Ophthalmology, New York University School of Medicine, 462 First Avenue - NBV 5N18, New York, NY 10016, USA.
Robert PostDepartment of Ophthalmology, New York University School of Medicine, 462 First Avenue - NBV 5N18, New York, NY 10016, USA.
Ansh JohriDepartment of Ophthalmology, New York University School of Medicine, 462 First Avenue - NBV 5N18, New York, NY 10016, USA.
Michele D LeeDepartment of Ophthalmology, New York University School of Medicine, 462 First Avenue - NBV 5N18, New York, NY 10016, USA.
Zsolt AblonczyDepartment of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue MSC676, Charleston, SC 29425, USA.
Christine A CurcioDepartment of Ophthalmology, University of Alabama at Birmingham School of Medicine, 1670 University Boulevard, Volker Hall Room 360, Birmingham, AL 35294, USA.
Thomas AchDepartment of Ophthalmology, University of Alabama at Birmingham School of Medicine, 1670 University Boulevard, Volker Hall Room 360, Birmingham, AL 35294, USA.
Paul SajdaDepartment of Biomedical Engineering, Columbia University, 500 West 120th Street, 351 Engineering Terrace, New York, NY 10027, USA.

Funding

Validated Autofluorescence in Age-Related Macular DegenerationR01EY015520 · NEI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI SMITH, ROLAND THEODORE · 2005 to 2018
$6.0M
QUANTITATIVE ANAYLSIS OF AGING RETINAR01EY006109 · NEI · UNIVERSITY OF WASHINGTON · PI CURCIO, CHRISTINE A · 1986 to 2012
$3.5M
Hyperspectral Imaging of the Normal and Age-related Macular Degeneration FundusR01EY021470 · NEI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI FAWZI, AMANI A, SMITH, ROLAND THEODORE · 2011 to 2014
$3.4M
The Mechanism of the Outer Blood-Retina Barrier BreakdownR01EY019065 · NEI · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI ABLONCZY, ZSOLT · 2010 to 2014
$1.7M
NEI NIH HHS R01 EY006109NEI NIH HHS R01 EY015520NEI NIH HHS R01 EY019065NEI NIH HHS R01 EY021470
6 · The paper itself

Abstract

Upon excitation with different wavelengths of light, biological tissues emit distinct but related autofluorescence signals. We used non-negative matrix factorization (NMF) to simultaneously decompose co-registered hyperspectral emission data from human retinal pigment epithelium/Bruch's membrane specimens illuminated with 436 and 480 nm light. NMF analysis was initialized with Gaussian mixture model fits and constrained to provide identical abundance images for the two excitation wavelengths. Spectra recovered this way were smoother than those obtained separately; fluorophore abundances more clearly localized within tissue compartments. These studies provide evidence that leveraging multiple co-registered hyperspectral emission data sets is preferential for identifying biologically relevant fluorophore information.

Indexed as

(100.2960) Image analysis(100.4145) Motion, hyperspectral image processing(110.4234) Multispectral and hyperspectral imaging(170.4470) Ophthalmology(170.6280) Spectroscopy, fluorescence and luminescence(170.6510) Spectroscopy, tissue diagnostics

Identifiers

PMID25574430
PMCPMC4285597

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.