ArticleBiomedical optics express2014
Automated method for the segmentation and morphometry of nerve fibers in large-scale CARS images of spinal cord tissue.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
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- Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration.Biophysical reviews · 2024Review
- AimSeg: A machine-learning-aided tool for axon, inner tongue and myelin segmentation.PLoS computational biology · 2023Article
- Automated pipeline for nerve fiber selection and g-ratio calculation in optical microscopy: exploring staining protocol variations.Frontiers in neuroanatomy · 2023Article
- The Need to Shift from Morphological to Structural Assessment for Carotid Plaque Vulnerability.Biomedicines · 2022Article
- Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices.Journal of visualized experiments : JoVE · 2022Article
- High-throughput segmentation of unmyelinated axons by deep learning.Scientific reports · 2022Article
- Tutorial: a computational framework for the design and optimization of peripheral neural interfaces.Nature protocols · 2020Review
- Transport and retention of artificial and real wastewater particles inside a bed of settled aerobic granular sludge assessed applying magnetic resonance imaging.Water research X · 2020Article
- Measurement-oriented deep-learning workflow for improved segmentation of myelin and axons in high-resolution images of human cerebral white matter.Journal of neuroscience methods · 2019Article
- AxonDeepSeg: automatic axon and myelin segmentation from microscopy data using convolutional neural networks.Scientific reports · 2018Article
- Lipid Order Degradation in Autoimmune Demyelination Probed by Polarized Coherent Raman Microscopy.Biophysical journal · 2017Article
- AxonPacking: An Open-Source Software to Simulate Arrangements of Axons in White Matter.Frontiers in neuroinformatics · 2017Article
- Axon and Myelin Morphology in Animal and Human Spinal Cord.Frontiers in neuroanatomy · 2017Review
- Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents.Brain plasticity (Amsterdam, Netherlands) · 2016Review
- Intravital assessment of myelin molecular order with polarimetric multiphoton microscopy.Scientific reports · 2016Article
- AxonSeg: Open Source Software for Axon and Myelin Segmentation and Morphometric Analysis.Frontiers in neuroinformatics · 2016Article
- Quantitative analysis of the myelin g-ratio from electron microscopy images of the macaque corpus callosum.Data in brief · 2015Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fully automated method for large-scale segmentation of nerve fibers from coherent anti-Stokes Raman scattering (CARS) microscopy images is presented. The method is specifically designed for CARS images of transverse cross sections of nervous tissue but is also suitable for use with standard light microscopy images. After a detailed description of the two-part segmentation algorithm, its accuracy is quantified by comparing the resulting binary images to manually segmented images. We then demonstrate the ability of our method to retrieve morphological data from CARS images of nerve tissue. Finally, we present the segmentation of a large mosaic of CARS images covering more than half the area of a mouse spinal cord cross section and show evidence of clusters of neurons with similar g-ratios throughout the spinal cord.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.