ArticleBioinformatics (Oxford, England)2015
Using isoelectric point to determine the pH for initial protein crystallization trials.
Article in Bioinformatics (Oxford, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Integrative machine learning approaches for enzyme kinetic parameter prediction.Briefings in bioinformatics · 2026Review
- Stability study of recombinant 9-valent human papillomavirus vaccine based onHuman vaccines & immunotherapeutics · 2025Article
- Intelligent Protein Design and Molecular Characterization Techniques: A Comprehensive Review.Molecules (Basel, Switzerland) · 2023Review
- Expression, purification, and characterization of self-assembly virus-like particles of capsid protein L1 HPV 52 in Pichia pastoris GS115.Journal, genetic engineering & biotechnology · 2023Article
- Terahertz phase shift sensing and identification of a chiral amino acid based on a protein-modified metasurface through the isoelectric point and peptide bonding.Biomedical optics express · 2023Article
- Solution characterization of the dynamic conjugative entry exclusion protein TraG.Structural dynamics (Melville, N.Y.) · 2022Article
- Glycosylated Zein Composite Nanoparticles for Efficient Delivery of Betulinic Acid: Fabrication, Characterization, and In Vitro Release Properties.Foods (Basel, Switzerland) · 2022Article
- Article
- Proteome-pI 2.0: proteome isoelectric point database update.Nucleic acids research · 2022Article
- Exploration of Streptococcus core genome to reveal druggable targets and novel therapeutics against S. pneumoniae.PloS one · 2022Article
- IPC 2.0: prediction of isoelectric point and pKa dissociation constants.Nucleic acids research · 2021Article
- Challenges in the Physical Characterization of Lipid Nanoparticles.Pharmaceutics · 2021Review
- Searching for conditions of protein self-assembly by protein crystallization screening method.Applied microbiology and biotechnology · 2021Article
- Article
- Antibody fragments structurally enable a drug-discovery campaign on the cancer target Mcl-1.Acta crystallographica. Section D, Structural biology · 2019Article
- The molecular mass and isoelectric point of plant proteomes.BMC genomics · 2019Article
- Proteome-pI: proteome isoelectric point database.Nucleic acids research · 2017Article
- Article
- IPC - Isoelectric Point Calculator.Biology direct · 2016Article
- Crystallization of nepenthesin I using a low-pH crystallization screen.Acta crystallographica. Section F, Structural biology communications · 2016Article
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Authors and funding
4 authors.
Funding
Abstract
motivationThe identification of suitable conditions for crystallization is a rate-limiting step in protein structure determination. The pH of an experiment is an important parameter and has the potential to be used in data-mining studies to help reduce the number of crystallization trials required. However, the pH is usually recorded as that of the buffer solution, which can be highly inaccurate.
resultsHere, we show that a better estimate of the true pH can be predicted by considering not only the buffer pH but also any other chemicals in the crystallization solution. We use these more accurate pH values to investigate the disputed relationship between the pI of a protein and the pH at which it crystallizes. AVAILABILITY AND IMPLEMENTATION: Data used to generate models are available as Supplementary Material. CONTACT: julie.wilson@york.ac.uk SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
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