ArticleMicrobiology (Reading, England)2015
(p)ppGpp modulates cell size and the initiation of DNA replication in Caulobacter crescentus in response to a block in lipid biosynthesis.
Article in Microbiology (Reading, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- A novel membrane stress response that blocks chromosomal replication by targeting the DnaA initiator via the ClpP protease.Journal of bacteriology · 2025Article
- Phosphate starvation decouples cell differentiation from DNA replication control in the dimorphic bacterium Caulobacter crescentus.PLoS genetics · 2023Article
- Mutant structure of metabolic switch protein in complex with monomeric c-di-GMP reveals a potential mechanism of protein-mediated ligand dimerization.Scientific reports · 2023Article
- Mutations inFrontiers in microbiology · 2022Article
- Many birds with one stone: targeting the (p)ppGpp signaling pathway of bacteria to improve antimicrobial therapy.Biophysical reviews · 2021Review
- A simple, quantitative assay for the detection of viable but non-culturable (VBNC) bacteria.STAR protocols · 2021Article
- Possible Roles for Basal Levels of (p)ppGpp: Growth Efficiency Vs. Surviving Stress.Frontiers in microbiology · 2020Review
- (p)ppGpp: Magic Modulators of Bacterial Physiology and Metabolism.Frontiers in microbiology · 2020Article
- Multilayered control of chromosome replication inBiochemical Society transactions · 2019Review
- Surface Area to Volume Ratio: A Natural Variable for Bacterial Morphogenesis.Trends in microbiology · 2018Article
- Bacterial Cell Size: Multifactorial and Multifaceted.Annual review of microbiology · 2017Review
- Shapeshifting to Survive: Shape Determination and Regulation in Caulobacter crescentus.Trends in microbiology · 2017Review
- Hit the right spots: cell cycle control by phosphorylated guanosines in alphaproteobacteria.Nature reviews. Microbiology · 2017Review
- A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues.Nature communications · 2016Article
- Adder and a coarse-grained approach to cell size homeostasis in bacteria.Current opinion in cell biology · 2016Review
- Article
- Topological control of the Caulobacter cell cycle circuitry by a polarized single-domain PAS protein.Nature communications · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Stress conditions, such as a block in fatty acid synthesis, signal bacterial cells to exit the cell cycle. Caulobacter crescentus FabH is a cell-cycle-regulated β-ketoacyl-acyl carrier protein synthase that initiates lipid biosynthesis and is essential for growth in rich media. To explore how C. crescentus responds to a block in lipid biosynthesis, we created a FabH-depletion strain. We found that FabH depletion blocks lipid biosynthesis in rich media and causes a cell cycle arrest that requires the alarmone (p)ppGpp for adaptation. Notably, basal levels of (p)ppGpp coordinate both a reduction in cell volume and a block in the over-initiation of DNA replication in response to FabH depletion. The gene ctrA encodes a master transcription factor that directly regulates 95 cell-cycle-controlled genes while also functioning to inhibit the initiation of DNA replication. Here, we demonstrate that ctrA transcription is (p)ppGpp-dependent during fatty acid starvation. CtrA fails to accumulate when FabH is depleted in the absence of (p)ppGpp due to a substantial reduction in ctrA transcription. The (p)ppGpp-dependent maintenance of ctrA transcription during fatty acid starvation initiated from only one of the two ctrA promoters. In the absence of (p)ppGpp, the majority of FabH-depleted cells enter a viable but non-culturable state, with multiple chromosomes, and are unable to recover from the miscoordination of cell cycle events. Thus, basal levels of (p)ppGpp facilitate C. crescentus' re-entry into the cell cycle after termination of fatty acid starvation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.