ArticleJournal of neuroinflammation2015
Retinal pericytes and cytomegalovirus infectivity: implications for HCMV-induced retinopathy and congenital ocular disease.
Article in Journal of neuroinflammation, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Associations between human cytomegalovirus infection and type 2 diabetes mellitus: a systematic review and meta-analysis.BMJ open · 2023Pooled it
- Molecular mechanisms of HCMV-driven vascular remodeling and its significance in cardiovascular diseases.Archives of microbiology · 2025Review
- Congenital cytomegalovirus retinitis of prematurity: a case report and literature review.Frontiers in pediatrics · 2025Article
- Article
- Human cytomegalovirus infection triggers a paracrine senescence loop in renal epithelial cells.Communications biology · 2024Article
- Ebola virus disrupts the inner blood-retinal barrier by induction of vascular endothelial growth factor in pericytes.PLoS pathogens · 2023Article
- Risk factors associated with Retinopathy of Prematurity development and progression.Scientific reports · 2022Article
- Roles of Interleukin-6-mediated immunometabolic reprogramming in COVID-19 and other viral infection-associated diseases.International immunopharmacology · 2022Review
- The emerging role of perivascular cells (pericytes) in viral pathogenesis.The Journal of general virology · 2021Review
- Extralesional microvascular and structural macular abnormalities in cytomegalovirus retinitis.Scientific reports · 2020Observational
- Unique method for human villous trophoblasts isolation from placental tissue explants.Clinical obstetrics, gynecology and reproductive medicine · 2020Article
- Multifaceted roles of pericytes in central nervous system homeostasis and disease.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Review
- Interactions between Amyloid-Β Proteins and Human Brain Pericytes: Implications for the Pathobiology of Alzheimer's Disease.Journal of clinical medicine · 2020Review
- Blocking VEGF signaling augments interleukin-8 secretionInternational journal of ophthalmology · 2020Article
- BK Virus Replication in the Glomerular Vascular Unit: Implications for BK Virus Associated Nephropathy.Viruses · 2019Article
- Human Vascular Pericytes and Cytomegalovirus Pathobiology.International journal of molecular sciences · 2019Review
- Mesangial cells, specialized renal pericytes and cytomegalovirus infectivity: Implications for HCMV pathology in the glomerular vascular unit and post-transplant renal disease.Journal of translational science · 2019Article
- Phosphorodiamidate morpholino targeting the 5' untranslated region of the ZIKV RNA inhibits virus replication.Virology · 2018Article
- SPECTRAL DOMAIN OPTICAL COHERENCE TOMOGRAPHY FINDINGS IN MACULA-INVOLVING CYTOMEGALOVIRUS RETINITIS.Retina (Philadelphia, Pa.) · 2018Observational
- Placental pericytes and cytomegalovirus infectivity: Implications for HCMV placental pathology and congenital disease.American journal of reproductive immunology (New York, N.Y. : 1989) · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHuman cytomegalovirus (HCMV) is the leading infectious cause of vision loss among congenitally infected children. Retinal pericytes play an essential role in maintaining retinal vascular and endothelial cell proliferation. However, the role of retinal pericytes in ocular HCMV pathogenesis is unknown.
methodsRetinal pericytes were exposed to clinical (SBCMV) and lab strains of HCMV; infectivity was analyzed by microscopy, immunofluorescence and qRT-PCR (reverse transcription polymerase chain reaction). Cytokine expression was examined by Luminex assay. Recombinant HCMV-GPF was used to examine viral replication kinetics. A Tricell culture model of the inner blood-retinal barrier (IBRB) was examined for cell type infectivity using immunohistochemistry.
resultsRetinal pericytes expressed the biomarker neuron-glial antigen 2. Antigenic expression profiles for several cytoskeletal, cell adhesion and inflammatory proteins were shared by both retinal and brain pericytes. Infected pericytes showed cytomegalic cytopathology and expressed mRNAs for the major immediate protein (MIE) and HCMV phosphorylated envelop protein 65. qRT-PCR analysis showed full lytic replication of HCMV in retinal pericytes. Pericytes exposed to SBCMV for 9 days expressed higher levels of vascular endothelial cell growth factor mRNA compared to controls. Luminex analysis of supernatants from SBCMV-infected retinal pericytes had increased levels of macrophage inflammatory protein-1α, beta-2 microglobulin (B2-m), matrix metalloproteinase-3 and -9 (MMP3/9), and lower levels of IL-6 and IL-8 compared to controls. At 24 hours post infection, pericytes expressed higher levels of IL-8, TIMP-1 (tissue inhibitor of metalloproteinase-1), and RANTES (regulated upon activation normal T cell-expressed and presumably secreted) but lower levels of MMP9. Time course analysis showed that both brain and retinal pericytes were more permissive for HCMV infection than other cellular components of the BBB (blood-brain barrier) and IBRB. Using a Tricell culture model of the IBRB (retinal endothelial, pericytes, Müller cells), retinal pericytes were most permissive for SBCMV infection. SBCMV infection of this IBRB Tricell mixture for 96 hours resulted in increased levels of IL-6, MMP9, and stem cell factor with a concomitant decrease in granulocyte-macrophage colony-stimulating factor and TNF-alpha.
conclusionIn retinal pericytes, HCMV induces proinflammatory and angiogenic cytokines. In the IBRB, pericytes likely serve as an amplification reservoir which contributes to retinal inflammation and angiogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.