ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2015
Giardia duodenalis-induced alterations of commensal bacteria kill Caenorhabditis elegans: a new model to study microbial-microbial interactions in the gut.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 56 citations in OpenAlex.
- The functional landscape of the appendix microbiome under conditions of health and disease.Gut pathogens · 2025Review
- The Influence of the ProtozoanInternational journal of molecular sciences · 2024Review
- A thermo-resistant and RNase-sensitive cargo fromJournal of extracellular biology · 2023Article
- Intelligent Biological Networks: Improving Anti-Microbial Resistance Resilience through Nutritional Interventions to Understand Protozoal Gut Infections.Microorganisms · 2023Review
- Regulatory Functions of Hypoxia in Host-Parasite Interactions: A Focus on Enteric, Tissue, and Blood Protozoa.Microorganisms · 2023Review
- Giardia hinders growth by disrupting nutrient metabolism independent of inflammatory enteropathy.Nature communications · 2023Article
- Gut Microbiota Crosstalk with Resident Macrophages and Their Role in Invasive Amebic Colitis and Giardiasis-Review.Microorganisms · 2023Article
- Gut microbiota biofilms: From regulatory mechanisms to therapeutic targets.The Journal of experimental medicine · 2023Article
- Gut Microbial Perturbation and Host Response Induce Redox Pathway Upregulation along the Gut-Liver Axis during Giardiasis in C57BL/6J Mouse Model.International journal of molecular sciences · 2023Article
- The Cysteine Protease Giardipain-1 fromInternational journal of molecular sciences · 2022Article
- Effects of Hydrogen Sulfide on the Microbiome: From Toxicity to Therapy.Antioxidants & redox signaling · 2022Review
- High-fat diet increases the severity of Giardia infection in association with low-grade inflammation and gut microbiota dysbiosis.Scientific reports · 2021Article
- Age andMicroorganisms · 2021Article
- Gastrointestinal biofilms in health and disease.Nature reviews. Gastroenterology & hepatology · 2021Review
- Natural Infection withmSphere · 2020Article
- Caenorhabditis elegans: a model to understand host-microbe interactions.Cellular and molecular life sciences : CMLS · 2020Review
- Review
- Review
- Cross-Domain and Viral Interactions in the Microbiome.Microbiology and molecular biology reviews : MMBR · 2019Review
- Pathobiont release from dysbiotic gut microbiota biofilms in intestinal inflammatory diseases: a role for iron?Journal of biomedical science · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardia duodenalis is the most common cause of parasitic diarrhea worldwide and a well-established risk factor for postinfectious irritable bowel syndrome. We hypothesized that Giardia-induced disruptions in host-microbiota interactions may play a role in the pathogenesis of giardiasis and in postgiardiasis disease. Functional changes induced by Giardia in commensal bacteria and the resulting effects on Caenorhabditis elegans were determined. Although Giardia or bacteria alone did not affect worm viability, combining commensal Escherichia coli bacteria with Giardia became lethal to C. elegans. Giardia also induced killing of C. elegans with attenuated Citrobacter rodentium espF and map mutant strains, human microbiota from a healthy donor, and microbiota from inflamed colonic sites of ulcerative colitis patient. In contrast, combinations of Giardia with microbiota from noninflamed sites of the same patient allowed for worm survival. The synergistic lethal effects of Giardia and E. coli required the presence of live bacteria and were associated with the facilitation of bacterial colonization in the C. elegans intestine. Exposure to C. elegans and/or Giardia altered the expression of 172 genes in E. coli. The genes affected by Giardia included hydrogen sulfide biosynthesis (HSB) genes, and deletion of a positive regulator of HSB genes, cysB, was sufficient to kill C. elegans even in the absence of Giardia. Our findings indicate that Giardia induces functional changes in commensal bacteria, possibly making them opportunistic pathogens, and alters host-microbe homeostatic interactions. This report describes the use of a novel in vivo model to assess the toxicity of human microbiota.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.