ArticleMolecular biotechnology2015
Cloning, characterization, and bacterial over-expression of an osmotin-like protein gene from Solanum nigrum L. with antifungal activity against three necrotrophic fungi.
Article in Molecular biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 34 citations in OpenAlex.
- Cloning and Expression Studies of Osmotin-Like Protein Gene from Solanum nigrum in Escherichia coli.Molecular biotechnology · 2026Article
- CtTLP13 Located in Extracellular Vesicles Enhances the Resistance of Safflower (Carthamus tinctorius) to Botrytis cinerea.Molecular plant pathology · 2026Article
- Natural SNP Variation in GbOSM1 Promotor Enhances Verticillium Wilt Resistance in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The potential of plant proteins as antifungal agents for agricultural applications.Synthetic and systems biotechnology · 2022Review
- Article
- Molecular Characterisation of Soybean Osmotins and Their Involvement in Drought Stress Response.Frontiers in genetics · 2021Article
- Genetic Response of Common Bean to the Inoculation with IndigenousJournal of fungi (Basel, Switzerland) · 2020Article
- Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants.Plants (Basel, Switzerland) · 2020Review
- TaTLP1 interacts with TaPR1 to contribute to wheat defense responses to leaf rust fungus.PLoS genetics · 2020Article
- Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus.Scientific reports · 2019Article
- Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.Transgenic research · 2018Article
- Xylem Sap Proteomics Reveals Distinct Differences BetweenFrontiers in microbiology · 2018Article
- Biotrophy-necrotrophy switch in pathogen evoke differential response in resistant and susceptible sesame involving multiple signaling pathways at different phases.Scientific reports · 2017Article
- Overexpression of a New Osmotin-Like Protein Gene (Frontiers in plant science · 2017Article
- Article
- Co-transformation of canola by chimeric chitinase and tlp genes towards improving resistance to Sclerotinia sclerotiorum.World journal of microbiology & biotechnology · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new osmotin-like protein gene from Solanum nigrum L. var indica (SindOLP) was cloned and overexpressed in Escherichia coli. The full-length intron-less gene is 744 bp, encoding a mature protein of 247 amino acids with a molecular mass of 26 kDa. The protein has an N-terminal cleavable signal sequence of 21 amino acids. There is the Thaumatin family signature pattern, with one each of amidation, N-myristoylation, casein kinase II phosphorylation, tyrosine kinase phosphorylation, and protein kinase C phosphorylation sites. Hydropathy plot showed that it has six transmembrane helices. It has antifungal activity and can permeabilize fungal hyphae and spores. SindOLP is most active at pH 8, 25 °C and its antifungal activity is retained after 75 °C for 30 min. SindOLP inhibits fungal spore germination. The protein however lacks glucanase activity. The potential for SindOLP in developing fungus-resistant, transgenic crops is discussed.
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