ArticleACS chemical biology2015
Genetic targeting of a small fluorescent zinc indicator to cell surface for monitoring zinc secretion.
Article in ACS chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 66 citations in OpenAlex.
- An Ultraresponsive Green Biosensor for Robust in Vivo Imaging of Synaptic Zinc Dynamics.ACS sensors · 2026Article
- hpGRISZ: a high-performance fluorescent biosensor for in vivo imaging of synaptic zinc dynamics.bioRxiv : the preprint server for biology · 2025Article
- Bright Zinc Probes with Thiomorpholine Monoxide Auxochromes for Imaging Insulin Secretion.Chemical & biomedical imaging · 2025Article
- The best of both worlds: Chemigenetic fluorescent sensors for biological imaging.Cell chemical biology · 2024Review
- Fine-tuned photochromic sulfonylureas for optical control of beta cell CaDiabetic medicine : a journal of the British Diabetic Association · 2023Article
- Chemical tags and beyond: Live-cell protein labeling technologies for modern optical imaging.Smart molecules : open access · 2023Review
- Article
- Organelle-Level Labile ZnACS sensors · 2022Article
- A Novel Imidazole Bound Schiff Base as Highly Selective "Turn-on" Fluorescence Sensor for ZnJournal of fluorescence · 2022Article
- Article
- Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021Review
- ZIGIR, a Granule-Specific ZnCell reports · 2020Article
- Functional analysis of islet cells in vitro, in situ, and in vivo.Seminars in cell & developmental biology · 2020Review
- Genetically Encoded, Photostable Indicators to Image Dynamic ZnAnalytical chemistry · 2019Article
- Optical tools for understanding the complexity of β-cell signalling and insulin release.Nature reviews. Endocrinology · 2018Review
- Fluorogen-activating proteins: beyond classical fluorescent proteins.Acta pharmaceutica Sinica. B · 2018Review
- Visualization of long-term MgChemical science · 2017Article
- Probes for monitoring regulated exocytosis.Cell calcium · 2017Review
- Single Fluorescent Protein-Based Indicators for Zinc Ion (Zn(2+)).Analytical chemistry · 2016Article
- eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging.ACS chemical biology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Numerous mammalian cells contain Zn2+ in their secretory granules. During secretion, Zn2+ is coreleased with granular cargos into extracellular medium so Zn2+ serves as a convenient surrogate marker for tracking the dynamics of secretion. Fluorescent Zn2+ sensors that can be selectively targeted to cells of interest would be invaluable tools for imaging Zn2+ release in multicellular systems including tissues and live animals. Exploiting the HaloTag labeling technology and using an optimized linker, we have engineered a fluorescent Zn2+ indicator that displayed a 15-fold fluorescence enhancement upon Zn2+ binding while reacting efficiently with a HaloTag enzyme in a cellular environment. Two-color imaging of ZIMIR-HaloTag and a red-emitting calcium indicator in pancreatic islet beta cells demonstrated that photoactivation of a channelrhodopsin was able to induce exocytosis of Zn2+/insulin granules and revealed heterogeneity in secretory activity along the cell membrane that was uncoupled from cellular Ca2+ activity. This integrated photonic approach for imaging and controlling the release of large dense core granules provides exquisite cellular selectivity and should facilitate future studies of stimulus-secretion coupling and paracrine signaling in secretory cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.