ArticleAmerican journal of respiratory and critical care medicine2015
House dust mites induce proliferation of severe asthmatic smooth muscle cells via an epithelium-dependent pathway.
Article in American journal of respiratory and critical care medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Bronchial smooth muscle extracellular vesicles interfere with bronchial epithelium metabolism and function in asthma.iScience · 2025Article
- From skin testing to molecular diagnostics: the precision leap in dust mite allergy diagnosis and clinical translation challenges.Frontiers in allergy · 2025Review
- Cysteinyl Leukotrienes in Allergic Inflammation.Annual review of pathology · 2025Review
- A novelThe European respiratory journal · 2024Article
- Commentary: Effect of curcumin nanoparticles on proliferation and migration of mouse airway smooth muscle cells and airway inflammatory infiltration.Frontiers in pharmacology · 2024Article
- Bronchial epithelial and airway smooth muscle cell interactions in health and disease.Heliyon · 2023Review
- Article
- Bronchial epithelia from adults and children: SARS-CoV-2 spread via syncytia formation and type III interferon infectivity restriction.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Protease-activated receptor-2: Role in asthma pathogenesis and utility as a biomarker of disease severity.Frontiers in medicine · 2022Review
- Insights Into Mucosal Innate Immune Responses in House Dust Mite-Mediated Allergic Asthma.Frontiers in immunology · 2020Review
- The Role of Dust Mites in Allergy.Clinical reviews in allergy & immunology · 2019Review
- The Aftermath of Bronchoconstriction.Journal of engineering and science in medical diagnostics and therapy · 2019Review
- Bitter Taste Receptors for Asthma Therapeutics.Frontiers in physiology · 2019Article
- Asthmatic Bronchial Smooth Muscle Increases CCL5-Dependent Monocyte Migration in Response to Rhinovirus-Infected Epithelium.Frontiers in immunology · 2019Article
- Cysteinyl leukotriene receptor 1 expression identifies a subset of neutrophils during the antiviral response that contributes to postviral atopic airway disease.The Journal of allergy and clinical immunology · 2018Article
- TGF-β1 Signaling and Tissue Fibrosis.Cold Spring Harbor perspectives in biology · 2018Review
- Type 2 immunity in asthma.The World Allergy Organization journal · 2018Review
- New targets for resolution of airway remodeling in obstructive lung diseases.F1000Research · 2018Review
- 15-Deoxy-Delta-12,14-Prostaglandin JFrontiers in immunology · 2017Article
- Nasal airway epithelial cell IL-6 and FKBP51 gene expression and steroid sensitivity in asthmatic children.PloS one · 2017Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
rationaleAsthma is a frequent airway disease, and asthma control determinants have been associated with indoor allergen sensitization. The most frequent allergens are house dust mites (HDM), which act in vivo on the bronchial epithelial layer. Severe asthma has also been associated with bronchial remodeling and more specifically with increased mass of bronchial smooth muscle (BSM). However, the relationship between HDM stimulation of the bronchial epithelial layer and BSM remodeling is unknown.
objectivesTo evaluate whether epithelial stimulation with HDM induces BSM cell proliferation in subjects with severe asthma.
methodsA total of 22 subjects with severe asthma and 27 subjects with no asthma were recruited. We have developed an in vitro culture model combining an epithelium layer in air-liquid interface (ALI) interacting with BSM. We assessed BSM proliferation using BrdU incorporation. We explored the role of epithelium-derived mediators using reverse-transcriptase polymerase chain reaction (RT-PCR) and ELISA in vitro and in vivo. Finally, leukotrienes receptor expression was assessed in vitro by flow cytometry and RT-PCR and ex vivo by laser microdissection and RT-PCR. MEASUREMENTS AND MAIN
resultsWe found that epithelial stimulation by HDM selectively increased the proliferation of asthmatic BSM cells and not that of nonasthmatic cells. The mechanism involved epithelial protease-activated receptor-2-dependent production of leukotrienes C4 associated with an overexpression of leukotrienes receptor CysLTR1 by asthmatic BSM cells in vitro and ex vivo.
conclusionsThis work demonstrates the selective role of HDM on BSM remodeling in patients with severe asthma and points out different therapeutic targets at epithelial and smooth muscle levels.
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