ArticleNucleus (Austin, Tex.)2015
The distinct functions of CENP-C and CENP-T/W in centromere propagation and function in Xenopus egg extracts.
Article in Nucleus (Austin, Tex.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 18 citations in OpenAlex.
- The CENP-A chaperone complex spatially organizes centromeres.Science advances · 2026Article
- The CENP-A chaperone complex spatially organizes centromeres.bioRxiv : the preprint server for biology · 2025Article
- Functional framework of the kinetochore and spindle assembly checkpoint in Arabidopsis.Plant physiology · 2025Article
- Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.Current biology : CB · 2025Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.Molecular biology of the cell · 2021Article
- Biochemical evidence for diverse strategies in the inner kinetochore.Open biology · 2020Review
- Enrichment of Aurora B kinase at the inner kinetochore controls outer kinetochore assembly.The Journal of cell biology · 2019Article
- Holliday junction recognition protein interacts with and specifies the centromeric assembly of CENP-T.The Journal of biological chemistry · 2019Article
- Distinct Roles of the Chromosomal Passenger Complex in the Detection of and Response to Errors in Kinetochore-Microtubule Attachment.Developmental cell · 2017Article
- Bub1 targeting to centromeres is sufficient for Sgo1 recruitment in the absence of kinetochores.Chromosoma · 2017Article
- Kinetochore assembly and function through the cell cycle.Chromosoma · 2016Review
- Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster.Open biology · 2016Article
- Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability.Open biology · 2016Article
- Article
- CENP-C is a blueprint for constitutive centromere-associated network assembly within human kinetochores.The Journal of cell biology · 2015Article
- Review
- The Power of Xenopus Egg Extract for Reconstitution of Centromere and Kinetochore Function.Progress in molecular and subcellular biologyReview
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The centromere is the chromosomal region in which the kinetochore is assembled to orchestrate chromosome segregation. It is defined by the presence of a histone H3 variant called Centromere Protein A (CENP-A) or CenH3. Propagation of centromere identity entails deposition of new CENP-A upon exit from mitosis in vertebrate cells. A group of 16 proteins that co-immunoprecipitate with CENP-A, the Constitutive Centromere Associated Network or CCAN, contribute to kinetochore assembly and function. For most of them it is still unclear how and when they are recruited to centromeres and whether they have a role in CENP-A deposition. Taking advantage of the Xenopus egg cell-free system, we have addressed these issues for CCAN proteins CENP-C, CENP-T and CENP-W. CENP-C recruitment occurs as soon as sperm DNA, containing CENP-A, is added to the egg extract, and continues after de novo incorporation of CENP-A in early interphase. In contrast, centromeric recruitment of CENP-T occurs in late interphase and precedes that of CENP-W, which occurs in mitosis. Unlike CENP-C, CENP-T and CENP-W do not participate in CENP-A deposition. However, like CENP-C, they play a major role in kinetochore assembly. Depletion of CENP-C results in reduced amount of CENP-T at centromeres, an effect more prominent in mitosis than in interphase. In spite of this, kinetochores can still be assembled under this condition although the recruitment of Ndc80 and Mis12 is decreased. Our results support the existence of 2 pathways for kinetochore assembly directed by CENP-C and CENP-T/W, which can be reconstituted in Xenopus egg extracts.
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