ArticlePLoS pathogens2015
Preferential use of central metabolism in vivo reveals a nutritional basis for polymicrobial infection.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 106 citations in OpenAlex.
- Selective sugar transport supports Proteus mirabilis fitness in the urinary tract.PLoS pathogens · 2026Article
- Effect of metabolites on the survival and biofilm formation of Pantoea piersonii (Basionym: Kalamiella piersonii) in synthetic urine media.BMC microbiology · 2026Article
- Glucose alters the evolutionary response to gentamicin in uropathogenicMicrobiology (Reading, England) · 2025Article
- Multi-omics investigation reveals unique markers inFrontiers in microbiology · 2025Article
- An Overview of the Association of the Urinary Tract Microbiome with Various Diseases and Implications for Therapeutics.Mini reviews in medicinal chemistry · 2025Review
- Development of a suite ofApplied and environmental microbiology · 2024Article
- Article
- Emerging roles for ABC transporters as virulence factors in uropathogenicProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Role of Metabolomics and Microbiology in Urinary Tract Infection.International journal of molecular sciences · 2024Review
- Dinner date: Neisseria gonorrhoeae central carbon metabolism and pathogenesis.Emerging topics in life sciences · 2024Review
- Organ agar serves as physiologically relevant alternative forInfection and immunity · 2023Article
- Defining the Roles of Pyruvate Oxidation, TCA Cycle, and Mannitol Metabolism in Methicillin-Resistant Staphylococcus aureus Catheter-Associated Urinary Tract Infection.Microbiology spectrum · 2023Article
- Molecular Typing ofJournal of pharmacy & bioallied sciences · 2023Article
- Peripheral metabolic alterations associated with pathological manifestations of Parkinson's disease in gut-brain axis-based mouse model.Frontiers in molecular neuroscience · 2023Article
- Phenotypic Assessment of Clinical Escherichia coli Isolates as an Indicator for Uropathogenic Potential.mSystems · 2022Article
- Essential Fitness Repertoire of Staphylococcus aureus during Co-infection with Acinetobacter baumanniimSystems · 2022Article
- Recurrent urinary tract infection and estrogen shape the taxonomic ecology and function of the postmenopausal urogenital microbiome.Cell reports. Medicine · 2022Article
- Article
- Glycosuria Alters Uropathogenic Escherichia coli Global Gene Expression and Virulence.mSphere · 2022Article
- Enhanced Antibiotic Tolerance of anMicroorganisms · 2022Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The human genitourinary tract is a common anatomical niche for polymicrobial infection and a leading site for the development of bacteremia and sepsis. Most uncomplicated, community-acquired urinary tract infections (UTI) are caused by Escherichia coli, while another bacterium, Proteus mirabilis, is more often associated with complicated UTI. Here, we report that uropathogenic E. coli and P. mirabilis have divergent requirements for specific central pathways in vivo despite colonizing and occupying the same host environment. Using mutants of specific central metabolism enzymes, we determined glycolysis mutants lacking pgi, tpiA, pfkA, or pykA all have fitness defects in vivo for P. mirabilis but do not affect colonization of E. coli during UTI. Similarly, the oxidative pentose phosphate pathway is required only for P. mirabilis in vivo. In contrast, gluconeogenesis is required only for E. coli fitness in vivo. The remarkable difference in central pathway utilization between E. coli and P. mirabilis during experimental UTI was also observed for TCA cycle mutants in sdhB, fumC, and frdA. The distinct in vivo requirements between these pathogens suggest E. coli and P. mirabilis are not direct competitors within host urinary tract nutritional niche. In support of this, we found that co-infection with E. coli and P. mirabilis wild-type strains enhanced bacterial colonization and persistence of both pathogens during UTI. Our results reveal that complementary utilization of central carbon metabolism facilitates polymicrobial disease and suggests microbial activity in vivo alters the host urinary tract nutritional niche.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.