Evidence map›Paper›PMID 25568341›Full record

ArticleMolecular biology of the cell2015

Kif2a depletion generates chromosome segregation and pole coalescence defects in animal caps and inhibits gastrulation of the Xenopus embryo.

Gerald Eagleson, Katherine Pfister, Anne L Knowlton, Paul Skoglund, Ray Keller, P Todd Stukenberg

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Wnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  5. Arf GAPs and molecular motors.Small GTPases · 2019
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Gerald EaglesonDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908.
Katherine PfisterDepartment of Biology, University of Virginia, Charlottesville, VA 22904.
Anne L KnowltonDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908.
Paul SkoglundDepartment of Biology, University of Virginia, Charlottesville, VA 22904.
Ray KellerDepartment of Biology, University of Virginia, Charlottesville, VA 22904.
P Todd StukenbergDepartment of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908 pts7h@virginia.edu.
University of Virginia · US

Funding

TRAINING IN CELL AND MOLECULAR BIOLOGYT32GM008136 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI STUKENBERG, P. TODD · 1985 to 2020
$8.5M
Dissection of the Inner Centromere Regulatory NetworkR01GM063045 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI BEKIRANOV, STEFAN, STUKENBERG, P. TODD · 2001 to 2013
$4.0M
Production, Validation and Distribution of the Xenopus ORFeomeR01HD069352 · NICHD · UNIVERSITY OF VIRGINIA · PI GILCHRIST, MICHAEL JAMES, HILL, DAVID E. · 2011 to 2015
$3.6M
Regulation of Axial Extension in Vertebrate EmbryosR01GM099108 · NIGMS · UNIVERSITY OF VIRGINIA · PI SKOGLUND, PAUL MICHAEL · 2012 to 2015
$1.2M
NICHD NIH HHS HD069352NICHD NIH HHS R01 HD069352NIGMS NIH HHS GM063045NIGMS NIH HHS GM099108NIGMS NIH HHS R01 GM063045NIGMS NIH HHS R01 GM099108NIGMS NIH HHS T32 GM008136
6 · The paper itself

Abstract

Kif2a is a member of the kinesin-13 microtubule depolymerases, which tightly regulate microtubule dynamics for many cellular processes. We characterized Kif2a depletion in Xenopus animal caps and embryos. Kif2a depletion generates defects in blastopore closure. These defects are rescued by removing the animal cap, suggesting that Kif2a-depleted animal caps are not compliant enough to allow gastrulation movements. Gastrulation defects are not rescued by a Kif2a mutated in an Aurora kinase phosphorylation site, suggesting that the phenotypes are caused by problems in mitosis. During animal cap mitoses, Kif2a localizes to the spindle poles and centromeres. Depletion of Kif2a generated multipolar spindles in stage 12 embryos. Kif2a-depleted animal caps have anaphase lagging chromosomes in stage 9 and 10 embryos and subsequent cytokinesis failure. Later divisions have greater than two centrosomes, generating extra spindle poles. Kif2a-depleted embryos are also defective at coalescing extra spindle poles into a bipolar spindle. The gastrulation and mitotic phenotypes can be rescued by either human Kif2a or Kif2b, which suggests that the two homologues redundantly regulate mitosis in mammals. These studies demonstrate that defects in mitosis can inhibit large-scale developmental movements in vertebrate tissues.

Indexed as

AnimalsChromosome SegregationCytokinesisDown-RegulationGastrulationHumansKinesinsSpindle PolesXenopus laevisXenopus ProteinsKIF2A protein, humanKIF2B protein, humanKIF2 protein, XenopusKinesinsXenopus Proteins

Identifiers

PMID25568341
PMCPMC4342028
OpenAlexW2115233052

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.