ArticleMolecular biology of the cell2015
Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Synaptogenic Assays Using Primary Neurons Cultured on Micropatterned Substrates.Methods in molecular biology (Clifton, N.J.) · 2025Article
- WWC1/2 regulate spinogenesis and cognition in mice by stabilizing AMOT.Cell death & disease · 2023Article
- EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in neurons.The Journal of cell biology · 2023Article
- Förster resonance energy transfer efficiency of the vinculin tension sensor in cultured primary cortical neuronal growth cones.Neurophotonics · 2022Article
- Flying under the radar: CDH2 (N-cadherin), an important hub molecule in neurodevelopmental and neurodegenerative diseases.Frontiers in neuroscience · 2022Review
- Mice with cleavage-resistant N-cadherin exhibit synapse anomaly in the hippocampus and outperformance in spatial learning tasks.Molecular brain · 2021Article
- FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins.Scientific reports · 2020Article
- Interaction of Tau, IL-6 and mitochondria on synapse and cognition following sevoflurane anesthesia in young mice.Brain, behavior, & immunity - health · 2020Article
- Kainate Receptor Activation Shapes Short-Term Synaptic Plasticity by Controlling Receptor Lateral Mobility at Glutamatergic Synapses.Cell reports · 2020Article
- Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization.Frontiers in cellular neuroscience · 2019Article
- The Emerging Role of Mechanics in Synapse Formation and Plasticity.Frontiers in cellular neuroscience · 2018Review
- Article
- The equilibrium between antagonistic signaling pathways determines the number of synapses in Drosophila.PloS one · 2017Article
- Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells.Nature communications · 2016Article
- Organization and dynamics of the actin cytoskeleton during dendritic spine morphological remodeling.Cellular and molecular life sciences : CMLS · 2016Review
- Actin Out: Regulation of the Synaptic Cytoskeleton.The Journal of biological chemistry · 2015Review
- Two-tiered coupling between flowing actin and immobilized N-cadherin/catenin complexes in neuronal growth cones.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The morphology of neuronal dendritic spines is a critical indicator of synaptic function. It is regulated by several factors, including the intracellular actin/myosin cytoskeleton and transcellular N-cadherin adhesions. To examine the mechanical relationship between these molecular components, we performed quantitative live-imaging experiments in primary hippocampal neurons. We found that actin turnover and structural motility were lower in dendritic spines than in immature filopodia and increased upon expression of a nonadhesive N-cadherin mutant, resulting in an inverse relationship between spine motility and actin enrichment. Furthermore, the pharmacological stimulation of myosin II induced the rearward motion of actin structures in spines, showing that myosin II exerts tension on the actin network. Strikingly, the formation of stable, spine-like structures enriched in actin was induced at contacts between dendritic filopodia and N-cadherin-coated beads or micropatterns. Finally, computer simulations of actin dynamics mimicked various experimental conditions, pointing to the actin flow rate as an important parameter controlling actin enrichment in dendritic spines. Together these data demonstrate that a clutch-like mechanism between N-cadherin adhesions and the actin flow underlies the stabilization of dendritic filopodia into mature spines, a mechanism that may have important implications in synapse initiation, maturation, and plasticity in the developing brain.
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