ArticleMolecular biology of the cell2015
A mechanism of leading-edge protrusion in the absence of Arp2/3 complex.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- A continuous-discrete model of cell contraction incorporating actin and intermediate filaments.iScience · 2026Article
- Polarized SOS activity orchestrates FGF-directed cell migration in vivo.Current biology : CB · 2026Article
- Electrospun Parallel, Crossed Fibers for Promoting Cell Adhesion and Migration.Materials (Basel, Switzerland) · 2025Article
- The Arp2/3 complex enhances cell migration on elastic substrates.Molecular biology of the cell · 2023Article
- Curved Nanofiber Network Induces Cellular Bridge Formation to Promote Stem Cell Mechanotransduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- p53 Related Protein Kinase is Required for Arp2/3-Dependent Actin Dynamics of Hemocytes inFrontiers in cell and developmental biology · 2022Article
- Review
- Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments.Nature communications · 2021Article
- Regulation of the Migration of Distinct Dendritic Cell Subsets.Frontiers in cell and developmental biology · 2021Review
- Induced Arp2/3 Complex Depletion Increases FMNL2/3 Formin Expression and Filopodia Formation.Frontiers in cell and developmental biology · 2021Article
- WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge.Molecular biology of the cell · 2020Article
- Quantitative Phase Imaging of Spreading Fibroblasts Identifies the Role of Focal Adhesion Kinase in the Stabilization of the Cell Rear.Biomolecules · 2020Article
- Differential Contributions of Actin and Myosin to the Physical Phenotypes and Invasion of Pancreatic Cancer Cells.Cellular and molecular bioengineering · 2020Article
- Loss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity.The Journal of clinical investigation · 2019Article
- Actin dynamics in cell migration.Essays in biochemistry · 2019Review
- Control of adhesion and protrusion in cell migration by Rho GTPases.Current opinion in cell biology · 2019Review
- Dynamins 2 and 3 control the migration of human megakaryocytes by regulating CXCR4 surface expression and ITGB1 activity.Blood advances · 2018Article
- Does self-organized criticality drive leading edge protrusion?Biophysical reviews · 2018Review
- Bimodal sensing of guidance cues in mechanically distinct microenvironments.Nature communications · 2018Article
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Authors and funding
8 authors.
Funding
Abstract
Cells employ protrusive leading edges to navigate and promote their migration in diverse physiological environments. Classical models of leading-edge protrusion rely on a treadmilling dendritic actin network that undergoes continuous assembly nucleated by the Arp2/3 complex, forming ruffling lamellipodia. Recent work demonstrated, however, that, in the absence of the Arp2/3 complex, fibroblast cells adopt a leading edge with filopodia-like protrusions (FLPs) and maintain an ability to move, albeit with altered responses to different environmental signals. We show that formin-family actin nucleators are required for the extension of FLPs but are insufficient to produce a continuous leading edge in fibroblasts lacking Arp2/3 complex. Myosin II is concentrated in arc-like regions of the leading edge in between FLPs, and its activity is required for coordinated advancement of these regions with formin-generated FLPs. We propose that actomyosin contraction acting against membrane tension advances the web of arcs between FLPs. Predictions of this model are verified experimentally. The dependence of myosin II in leading-edge advancement helps explain the previously reported defect in directional movement in the Arpc3-null fibroblasts. We provide further evidence that this defect is cell autonomous during chemotaxis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.