ArticleGenome research2015
A large-scale, in vivo transcription factor screen defines bivalent chromatin as a key property of regulatory factors mediating Drosophila wing development.
Article in Genome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Transcription Factor Evolution Associated with Social Transitions in Blattodea.Journal of molecular evolution · 2026Article
- SP1 antagonizes H3K27me3 to shape chromatin landscapes for RNA polymerase II recruitment during gastrulation.Nucleic acids research · 2026Article
- Recombinant inbred line panels inform the genetic architecture and interactions of adaptive traits in Drosophila melanogaster.G3 (Bethesda, Md.) · 2025Article
- The histone demethylase dLsd1 regulates organ size by silencing transposable elements.Communications biology · 2025Article
- LKRSDH-dependent histone modifications of insulin-like peptide sites contribute to age-related circadian rhythm changes.Nature communications · 2024Article
- PARP-1 is a transcriptional rheostat of metabolic and bivalent genes during development.Life science alliance · 2024Article
- Twenty-seven ZAD-ZNF genes of Drosophila melanogaster are orthologous to the embryo polarity determining mosquito gene cucoid.PloS one · 2023Article
- Evolutionary changes in gene expression profiles associated with the coevolution of male and female genital parts among closely related ground beetle species.BMC genomics · 2022Article
- NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization.Nucleic acids research · 2022Article
- Tagging Drosophila Proteins with Genetically Encoded Fluorophores.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Three classes of epigenomic regulators converge to hyperactivate the essential maternal gene deadhead within a heterochromatin mini-domain.PLoS genetics · 2022Article
- Developmental Robustness: The Haltere Case inFrontiers in cell and developmental biology · 2021Article
- bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage.PLoS genetics · 2020Article
- Innovation of heterochromatin functions drives rapid evolution of essential ZAD-ZNF genes ineLife · 2020Article
- Limited Availability of General Co-Repressors Uncovered in an Overexpression Context during Wing Venation inGenes · 2020Article
- Dynamic Competition of Polycomb and Trithorax in Transcriptional Programming.Annual review of biochemistry · 2020Review
- A parallelized, automated platform enabling individual or sequential ChIP of histone marks and transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Large-Scale TransgenicGenetics · 2020Article
- Expression of E93 provides an instructive cue to control dynamic enhancer activity and chromatin accessibility during development.Development (Cambridge, England) · 2020Article
- Genetic interaction screen for severe neurodevelopmental disorders reveals a functional link between Ube3a and Mef2 in Drosophila melanogaster.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Transcription factors (TFs) are key regulators of cell fate. The estimated 755 genes that encode DNA binding domain-containing proteins comprise ∼ 5% of all Drosophila genes. However, the majority has remained uncharacterized so far due to the lack of proper genetic tools. We generated 594 site-directed transgenic Drosophila lines that contain integrations of individual UAS-TF constructs to facilitate spatiotemporally controlled misexpression in vivo. All transgenes were expressed in the developing wing, and two-thirds induced specific phenotypic defects. In vivo knockdown of the same genes yielded a phenotype for 50%, with both methods indicating a great potential for misexpression to characterize novel functions in wing growth, patterning, and development. Thus, our UAS-TF library provides an important addition to the genetic toolbox of Drosophila research, enabling the identification of several novel wing development-related TFs. In parallel, we established the chromatin landscape of wing imaginal discs by ChIP-seq analyses of five chromatin marks and RNA Pol II. Subsequent clustering revealed six distinct chromatin states, with two clusters showing enrichment for both active and repressive marks. TFs that carry such "bivalent" chromatin are highly enriched for causing misexpression phenotypes in the wing, and analysis of existing expression data shows that these TFs tend to be differentially expressed across the wing disc. Thus, bivalently marked chromatin can be used as a marker for spatially regulated TFs that are functionally relevant in a developing tissue.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.